1995 Fiscal Year Final Research Report Summary
Effects of platelets and coagulation factors on aortic smooth cell proliferation
| Project/Area Number |
06670236
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Miyazaki Medical College |
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Principal Investigator |
SUMIYOSHI Akinobu Miyazaki Medical College, Department of Pathology, Professor, 医学部, 教授 (80038695)
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| Co-Investigator(Kenkyū-buntansha) |
KISANUKI Atsushi Miyazaki Medical College, Dept.Pathol., Research associate, 医学部, 助手 (70253846)
MARUTSUKA Kousuke Miyazaki Medical College, Dept.Pathol., Research associate, 医学部, 助手 (00239154)
ASADA Yujiro Miyazaki Medical College, Dept.Patol., Associate professor, 医学部, 助教授 (70202588)
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| Project Period (FY) |
1994 – 1995
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| Keywords | aortic smooth muscle cells / neointima / platelets / tissue factor |
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| Research Abstract |
We investigated the effects of platelets and tissue factor (TF) on proliferation and migration of rabbit aortic smooth muscle cells (SMCs) in vivo and in vitro. Platelet adhesion and aggregation were present on the aortic subendothelium after balloon injury. Subsequently migration and proliferation of SMCs were observed and the neointima was produced. Immunohistochemical studies showed that almost all of SMCs in the neointima were positive for TF.Balloon injury on the neointima induced fibrin-rich large thrombi and the SMC proliferation. TF showed a strong chemotactic migration activity for cultured aortic SMCs. The migration ability of TF was comparable to those of platelet-derived growth factor (PDGF)-BB and basic fibroblast-growth factor (bFGF). On the other hand, TF did not induce the SMC proliferation. These results indicate that adhered and/or aggregated platelets could contribute to SMC proliferation and migration immediately after balloon injury, furthermore, TF is not only a coagulation factor but also a chemotactic factor for vascular SMCs, and suggest that platelets and TF could play an important role in atherogenesis as well as in hemostasis and thrombosis.
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