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1995 Fiscal Year Final Research Report Summary

Aberrant function and expression of gap junction proteins (connexins) during carcinogenesis

Research Project

Project/Area Number 06670238
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionSapporo Medical University School of Medicine

Principal Investigator

OYAMADA Masahito  Sapporo Medical University, School of Medicine, Department of Pathology, Assistant Professor, 医学部, 講師 (30183255)

Co-Investigator(Kenkyū-buntansha) OYAMADA Yumiko  Sapporo Medical University, School of Medicine, Department of Pathology, Instruc, 医学部, 助手 (40231245)
Project Period (FY) 1994 – 1995
KeywordsIntercellular communication / Gap junctions / Connexin / Multistage carcinogenesis / Mouse skin carcinogenesis / Esophageal carcinoma / Tumor promoter / Hamster tongue carcinogenesis
Research Abstract

(1) Aberrant expression of gap junction proteins (connexins[Cx]) during multistage mouse skin carcinogenesis (Carcinogenesis 16 : 1287-1297,1995)
(a) Cx26 and Cx43 were differentially expressed in normal and surrounding non-tumorous epidermis. (b) In papillomasCx26 and Cx43 were frequently co-localized in the same gap junction plaques. (c) In squamous cell carcinomas, the expression of both Cx26 and Cx43 significantly decreased compared with surrounding non-tumourous epidermis. (d) The expression of Cx26 was reduced as cancer cells became morphologically less differentiated. (e) Squamous cell carcinomas at invasiv sites showed clear reduction of Cx26 and Cx43. (f) In squamous cell carcinomas metastasized into lymhph nodes, Cx26 was expressed, but few carcinoma cells expressed Cx43. (g) The localization of E-cadherin on the plasma membrane between cancer cells was maintained even at invasive and metastatic sites.
(2) Aberrant expression, function and localization of connexins in human esophageal carcinoma cell lines (J Cancer Res Clin Oncol 120 : 445-453,1994)
(a) Normal human esophageal tissue expressed both Cx26 and Cx43. (b) Most of the human esophageal carcinoma cell lines expressed lower amounts of Cx26 and Cx43 mRNAs than normal human esophageal tissues. (c) The co-expression of Cx26 and Cx43 mRNAs and proteins was observed only in two cell lines that showed a high level of GJIC and non-progressive tumor development. (d) E-cadherin was expressed in all cell lines.
(3) Effect of a tumour promoter, DDT,on hepatic gap junctional intercellular communication in rats (Carcinogenesis 15 : 517-542,1994)
DDT inhibited hepatic gap-junctional intercellular communication in vivo dose-dependently and changed Cx32 and Cx26 protein expression and localization.
(4) Changes in the expression of connexins in hamster oral epithelium during wounnd healing
During wound healing, the expression and localization of connexin proteins and transcripts were changed drastically.

  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Oyamada, Y., Oyamada, M. et al.: "Aberrant expression, function and localization of connexins in human esophageal carcinoma cell lines with different degrees of tumorigenicity." J Cancer Res Clin Oncol. 120. 445-453 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tateno, C,, Oyamada, M. et al.: "Effect of DDT on hepatic gap junctional intercellular communication in rats." Carcinogenesis. 15. 517-521 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oyamada, M., et al.: "The expression, phosphorylation,and localization of connexin 43 and gapjunctional intercellular communication during the establishment of a synchronized contraction of cultured neonatal rat cardiac myocytes." Exp Cell Res. 212. 351-358 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kojima, T., Oyamada, M. et al.: "Sequential changes of hepatic gap junctions during the course of acute liver injury and restoration after thioacetamide treatment" Virchows Archive A. 425. 407-412 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chiba, H., Oyamada, M. et al.: "Hormonal regulation of connexin 43 expression and gap junctional communication in human osteoblastic cells" Cell Struct Funct. 19. 173-177 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kojima, T., Oyamada, M. et al.: "Rapid appearance of connexin 26-positive gap-junctions in centrilobular hepatocytes without induction of mRNA and protein synthesis in isolated perfused liver of female rat" J Cell Sci. 107. 3579-3590 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kamibayashi, Y., Oyamada, M. et al.: "Aberrant expression of gap junction proteins(connexins)is associated with tumor progression during multistage mouse skin carcinogenesis" Carcinogenesis. 16. 1287-1297 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimura, H., Oyamada, M. et al.: "Reversible inhibition of gap junctional intercellular communication, synchronous contraction and synchronism of intracellular Ca 2+ fluctuation in cultured neonatal rat cardiac myocytes by heptanol" Exp Cell Res. 220. 348-356 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oyamada, M., et al.: "Expression of multiple connexins is differentially modulated during multistage hepatocarcinogenesis" Prog Cell Res. 4. 103-106 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimura, H., Oyamada, M. et al.: "Involvement of connexin43 localization and gap junctional intercellular communication in the establishment of a synchronized contraction of cultured neonatal rat cardiac myocytes" Prog Cell Res. 4. 155-158 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kamibayashi, Y., Oyamada, M. et al.: "Expression and localization of connexin 26 and 43 in mouse epidermis in vivo" Prog Cell Res. 4. 353-356 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mori, M., Oyamada, M. et al.: "Molecular structure and pathophysiological roles of intercellular junctions in the liver." Progress in Hepatology. 1. 1-14 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小山田正人他.: "ギャップ結合チャネル病" 細胞. 27. 276-280 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 榎本克彦,小山田正人他: "肝癌における細胞接着装置の動態-gap junction, tight junction-." 病理と臨床. 13. 940-946 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小山田正人他.: "ギャップ結合蛋白質(コネキシン)と腫瘍の悪性化" 細胞. (6月号予定).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小山田正人他.: "ギャップ結合蛋白質(コネキシン)と腫瘍の悪性化" 細胞. (6月号予定). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oyamada Y,Oyamada M,Fusco A,Yamasaki H.: "Aberrant expression, function and localization of connexins in human esophageal carcinoma cell lines with different degrees of tumorigenicity." J Cancer Res Clin Oncol. 120. 445-453 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tateno C,Ito S,Tanaka M,Oyamada M,Yoshitake A.: "Effect of DDT on hepatic gap junctional intercellular communication in rats." Carcinogenesis. 15. 517-521 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oyamada M,Kimura H,Oyamada Y,Miyamoto A,Ohshika H,Mori M.: "The expression, phosphorylation, and localization of connexin 43 and gap-junctional intercellular communication during the establishment of a synchronized contraction of cultured neonatal rat cardiac myocytes." Exp Cell Res. 212. 351-358 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kojima T,Sawada N,Zhong Y,Oyamada M,Mori M.: "Sequential changes in interecellular junctions between hepatocytes during the course of acute liver injury and restoration after thioacetamide treatment." Virchows Arch A. 425. 407-412 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kojima T,Sawada N,Oyamada M,Chiba H,Isomura H,Mori M.: "Rapid appearance of connexin 26-positive gap junctions in centrilobular hepatocytes without induction of mRNA and protein synthesis in isolated perfused liver of female rat." J Cell Sci. 107. 3579-3590 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chiba H,Sawada N,Oyamada M,Kojima T,Iba K,Ishii S,Mori M.: "Hormonal regulation of connexin 43 expression and gap junctional communication in human osteoblastic cells." Cell Struct Funct. 19. 173-177 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kamibayashi Y,Oyamada Y,Mori M,Oyamada M.: "Aberrant expression of gap junction proteins (connexins) is associated with tumor progression during multistage mouse skin carcinogenesis in vivo." Carcinogenesis. 16. 1287-1297 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura H,Oyamada Y,Ohshika H,Mori M,Oyamada M.: "Reversible inhibition of gap junctional intercellular communication, synchronous contraction, and synchronism of intracellular Ca2+ fluctuation in cultured neonatal rat cardiac myocytes by heptanol." Exp Cell Res. 220. 348-356 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oyamada M,Sakamoto H,Enomoto K,Oyamada Y,Kojima T,Sawada N,Mori M.: "Expression of multiple connexins is differentially modulated during multistage hepatocarcinogenesis." Prog Cell Res. 1. 103-106 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimura H,Oyamada M,Oyamada Y,Kamibayashi Y,Mori M,Ohshika H.: "Involvement of connexin43 localization and gap junctional intercellular communication in the establishment of a synchronized contraction of cultured neonatal rat cardiac myocytes." Prog Cell Res. 4. 155-158 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kamibayashi Y,Oyamada M,Oyamada Y,Abe S,Takahashi M,Mori M: "Expression and localization of connexin 26 and 43 in mouse epidermis in vivo." Prog Cell Res. 4. 353-356 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mori M,Zhong Y,Enomoto K,Oyamada M,Sawada N: "Molecular structure and pathophysiological roles of intercellular junctions in the liver." Progress in Hepatology. 1. 1-14 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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