1995 Fiscal Year Final Research Report Summary
Extinction of TCR/CD3 and lck gene expression in somatic cell hybrids.
| Project/Area Number |
06670245
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Sasaki Institute |
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Principal Investigator |
OIKAWA Tsuneyuki Sasaki Inst.Dep.Cell Genet., Head, 細胞遺伝部, 部長 (80150241)
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| Co-Investigator(Kenkyū-buntansha) |
KUBOTA Yoshiko Sasaki Inst.Dep.Cell Genet., Research Associate, 細胞遺伝部, 研究員 (30260102)
YAMADA Toshiyuki Sasaki Inst.Dep.Cell Genet., Research Associate, 細胞遺伝部, 研究員 (20183981)
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| Project Period (FY) |
1994 – 1995
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| Keywords | gene experssion / extinction / hybrid / T cell / TCR / lck |
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| Research Abstract |
The regulation of expression of cell type-specific genes during cell differentiation is a complex process involving interaction between multiple positive and negative reguratory factors. The somatic cell hybrid system has been used to study hierarchial negative regulation of cell type-specific genes.
We found that expression of the TCR/CD3 and lck genes is extinguished in T cell x fibroblast cell hybrids. In the hybrids, expression of the genes encoding lymphoid-specific transcription factors, such as LEF-1, TCF-1, GATA-3, Ikaros/LyF-1, Elf-1, Myb and Fli-1, is extinguished, while that of the genes encoding ubiquitously expressed transcription factors, such as Sp-1, Ets-2 and CREB,is not extinguished.
These results suggest that putative repressors in fibroblast shut off the expression of several lymphoid-specific transcription factors and consequently downregulate T cell specific structural gene expression.
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