1995 Fiscal Year Final Research Report Summary
Expression of human endogenous retrovirus gene in tumor cells
| Project/Area Number |
06670246
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Tokyo Metropolitan institute of Gerontology |
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Principal Investigator |
MARUYAMA Naoki Tokyo Metropolitan Institute of Gerontology, Department of Molecular Pathology Head of Department, 分子病理, 研究部長 (00115940)
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| Project Period (FY) |
1994 – 1995
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| Keywords | human endogenous retrovirus / LTR / promoter / DNA binding protein |
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| Research Abstract |
Human endogenous retrovirus genes are integrated in the chromosomal DNA and expressed by ordinal transcription factors.We investigated the promoter activity determined by LTR in human endogenous retrovirus gene Clone 4-1.By using luciferase assay as reporter gene and Jarkat cell we determined promoter fragment in the LTR of Clone 4-1.We determined the 184-bp fragment having promoter activity and 242-bp fragment having suppressive activity on the expression.We observed the presence of DNA binding protein to the 184-bp fragment by gel shift assay and in vivo foot printing assay.Our study suggested that the expression of human endogenous retrovirus gene is determined by a transcriptional element in LTR.Small numbers of DNA binding protein as transcriptional factors are involved in this process.
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[Publications] Nagasawa, R., Maruyama, N., Kubo, S., Yumura, W., Mitarai, T., Isoba, K.and Kanagawa, O.: "Induction of Ly-49 on interspecies hybridoma between differentiation stage specific murine T cell and rat T lymphoma" Cellular Immunology. 156. 180-190 (1994)
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[Publications] Kitamura, M., Maruyama, N., Shirasawa, T., Watanabe, K., Tateno, M.and Yoshiki, T.: "Expression of an endogenous retroviral gene product in human placenta." International J.Cancer. 58. 836-840 (1994)
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[Publications] Utsunomiya, Y., Ogura, M., Kawamura, T., Mitarai, T., Maruyama, N.and Sakai, O.: "Supperssion of immune complex nephritis in NZB/W F1 mice by a procycline analog." Clinical and Experimental Immunology. 99. 454-460 (1995)
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[Publications] Sekigawa, I., Tamura, N., Hishikawa.T., Iida, N., Hashimoto, H., Shimizu, K., Hirose, S., Kira, S., Maruyama, N.and Yamamoto, N.: "Detection of type C retroviral particles in the lung cells of a patient with systemic lupus erythematosus (in Japanese)." Japanese J.Inflammation. 15. 401-408 (1996)
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