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1995 Fiscal Year Final Research Report Summary

The analysis of role of calcium-dependent protein kinase in the mechanism of parasitism of malarial parasites

Research Project

Project/Area Number 06670256
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 寄生虫学(含医用動物学)
Research InstitutionKobe University

Principal Investigator

SAITO Atsuko (伊藤 あつ子)  Kobe University, School of Medicine, Research associate, 医学部, 助手 (00223131)

Co-Investigator(Kenkyū-buntansha) KONOSHI Eiji  Kobe University, School of Medicine, Research associate, 医学部, 助手 (40135786)
Project Period (FY) 1994 – 1995
KeywordsMalaria / Protein kinase / Calcium / Molecular cloning
Research Abstract

In 1994 : Polymerase chain reaction (PCR) was carried out against P.yoelii genomic DNA with oligonucleotide primers which were designed from highly conserved regions among calcium-dependent protein kinases (protein kinase C and Ca^<2+>/calmodulin-dependent protein kinase) of a wide range of organisms and a PCR product was yielded. P.yoelii genomic DNA library was screened using this PCR product as a primer. Sequencing of the insert of a positive clone revealed that it contained a gene which codes a protein kinase.
In 1995 : The comparison of the primary structure of the parasite protein kinase with those of various kinds of protein kinases of other organisms showed that the parasite protein belongs to cAMP-dependent protein kinase catalytic subunit family members. In situ gel assay of P.yoelii proteins showed that there are at least 8 kinds of protein kinases of P.yoelii. And the calcium-dependent protein kinase showed the highest activity from late trophozoite stage to schizont stage. The protein kinase which is more activated in the presence of EGTA showed the highest activity from schizont stage to early trophozoite stage. The study of the effect of various inhibitors of protein kinases on the growth of P.falciparum in vitro demonstrated that inhibitors of calcium-dependent protein kinases (protein kinase C and Ca^<2+>/calmodulin-dependent protein kinase) inhibit the growth of P.falciparum.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] Atsuko Saito-Ito: "Cloning and structual analysis of the gene for cAMP-dependent protein kinase catalytic subunit from Plasmodium yoelii" Biochimica et Biophysica Acta. 1269. 1-5 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Atsuko Saito-Ito, Shenyi He, Masatsugu Kimura, Takeo Matsumura, Kazuyuki Tanabe.: "Cloning and structual analysis of the gene for cAMP-dependent protein kinase catalytic subunit from Plasmodium yoelii" Biochimica et Biophysica Acta.1269. 1-5 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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