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1995 Fiscal Year Final Research Report Summary

Analysis of the gene that regulate production of Vibrio cholerae El Tor cytolysin

Research Project

Project/Area Number 06670291
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Bacteriology (including Mycology)
Research InstitutionOsaka University

Principal Investigator

YAMAMOTO Koichiro  Osaka Univ., Res.Inst.Microbial Dis., Associate Professor, 微生物病研究所, 助教授 (30158274)

Co-Investigator(Kenkyū-buntansha) IIDA Tetsuya  Osaka Univ., Res.Inst.Microbial Dis., Research Associate, 微生物病研究所, 助手 (90221746)
HONDA Takeshi  Osaka Univ., Res.Inst.Microbial Dis., Professor, 微生物病研究所, 教授 (60029808)
Project Period (FY) 1994 – 1995
KeywordsVibrio cholerae / Hemolysin / DNA sequence / Enterotoxin / Phospholipase / Cholera toxin / Gene / Protein
Research Abstract

From the Tn5-inserted mutant library of Vibrio cholerae O1, we found a mutant, NF404, which lost the production of both hemolysin and cholera toxin (CT) even though the Tn5-insertion site was out side from the structural genes for hemolysin and CT.Cloning and sequencing analysis of the homologous region from the wild-type strain, revealed that the sequence spanning the coding region of an ORF1 nominated as lypA,encoding a 39.5 kDa protein. Deduced amino acid sequence of the lypA gene had 37.6% identity to the lysophospholipase L2 of Escherichia coli. Introduction of lypA into NF404 has lost cholera toxin and hemolysin productions, restored the lysophospholipase activity and CT production but not the hemolytic activity. Inactivation of lypA of the wild-type strains by single crossover insertion decreased the lysophospholipase L2 activity and cholera toxin production but not the hemolytic activity. These defective strains decreased enterotoxicity. These results suggest that lypA is possibly required for the expression of CT and may play a role in pathogenicity of Vibrio cholerae.

  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Yamamoto K.,G.de Volle.,Xu M.,Miwatani T. and Honda T.: "Amino acids of the cholera toxin from Vibrio cholerae O37 strain S7 which differ from those of strain O1." Gene. 163. 155-156 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto K.Shrestha J.,Iida T.,Yoh M.,and Honda T.: "Molecular epidemiology of Vibrio cholerae O1 isolated in Nepad." J. Diarrhoeal Dis. Res.13. 113-117 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Honma Y.,Yamamoto K.,and Iwanaga M.: "Aberrant gene for El Tor hemolysin from Vibrio cholerae non-O1,NO37." FEMS Microbiol. Lett.133. 151-154 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagamune K.,Yamamoto K.and Honda T.: "Cloning of a new hemolysin gene of Vibrio cholerae O1." FEMS Microbiol. Lett.128. 265-269 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Naka A.,Yamamoto K.,and Honda T.: "Vibrio cholerae O139 produces a protease which is indistinguishable from the haemagglutinin/protease of Vibrio cholerae O1 and non-O1." FEMS Immunol. Med. Microbiol.11. 87-90 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Adnan S.,Yamamoto K.,Tojo H. and T. Honda.: "Cloning and expression of lysophospholipase L2 of Vibrio cholerae." Biophys. Biochim. Acta.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山本耕一郎: "病気のバイオサイエンス(担当:細菌ワクチン研究)" 大阪大学出版会, (8) (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Xu, M.et al.: "Demonstration and characterization of simultaneous production of a thermostable direct hemolysin (TDH/I) and a TDH-related hemolysin (TRHx) by a clinically-isolated Vibrio parahaemolyticus, TH3766." Infect.Immun.62. 166-171 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyagi, K.et al.: "Successful application of enzyme-labeled oligonucleotide probe for rapid and accurate cholera diagnosis in a clinical laboratory." Microbiol.Immunol.38. 301-304 (1994)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Shimaoka M.et al.: "Development of an enzyme-labeled oligonucleotide probe for detection of the mecA gene in methicillin-resistant Staphylococcus aureus." J.Clin.Microbiol.32. 1866-1869 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Xu, M.et al.: "Construction of an isogenic mutant Vibrio parahaemolyticus having a deletion in the thermostable direct hemolysin-related hemolysin gene (trh)." J.Bacteriol.176. 4757-4760 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tang, G.et al.: "A mutant toxin of Vibrio parahaemolyticus thermostable direct hemolysin which has lost hemolytic activity but retains ability to bind to erythrocytes." Infect.Immun.62. 3299-3304 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iida, T.et al.: "Isolation of mutant toxin of Vibrio parahaemolyticus hemolysin by in vitro mutagenesis." Toxicon. 33. 209-216 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taniguchi, T.et al.: "Evidence that the N-terminal amino acid sequence of pilus colonization factor antigen III produced by human enterotoxigenic Escherichia coli is similar to that of TcpA pilin of Vibrio cholerae." J.Infect.Dis.170. 1049-1050 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagayama, K.et al.: "Characterisation of a haemolysin related to Vp-TDH produced by a Kanagawa phenomenon-negative clinical isolate of Vibrio parahaemolyticus." J.Med.Microbiol.42. 83-90 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taniguchi, T.et al.: "Sequencing of the gene encoding the major pilin of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli and evidence that CFA/III is related to type IV pili." Infect.Immun.63. 724-278 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naka, A.et al.: "Vibrio cholerae O139 produces a protease which is indistinguishable from the haemagglutinin/protease of Vibrio cholerae O1 and non-O1." FEMS Immunol.Med.Microbiol.11. 87-90 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagamune, K.et al.: "Cloning of a new hemolysin gene of Vibrio cholerae O1." FEMS Microbiol.Lett.128. 265-269 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuyama, J.et al.: "Monoclonal antibody developed against a hemolysin of Bacillus thuringensis." Microbiol.Immunol.39. 619-622 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taniguchi, T.et al.: "Use of a new oligonucleotide probe for detection of colonization factor antigen III gene in enterotoxigenic Escherichia coli." Eur.J.Clin.Microbiol.Infect.Dis.14. 713-716 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuyama, J.et al.: "Monoclonal antibody developed against a hemolysin of Bacillus thuringiensis." Microbiol.Immunol.39. 619-622 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, K.et al.: "Amino acids of the cholera toxin from Vibrio cholerae O37 strain S7 which differ from those of strain O1." Gene. 163. 155-156 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto, K.et al.: "Molecular epidemiology of Vibrio cholerae Ol isolated in Nepal." J.Diarrhoeal Dis.Res.13. 113-117 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honma, Y.et al.: "Aberraant gene for El Tor hemolysin from vibrio cholerae non-Ol, NO37." FEMS Microbiol.Lett.133. 151-154 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Whayb, S.A.et al.: "Cloning and expression of lysophospholipase L_2 of Vibrio cholerae." Biophys.Biochim.Acta.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suthienkul, O.et al.: "Restriction fragment Length polymorphism of the tdh and trh genes in clinical Vibrio parahaemolyticus strains." J.Clin.Microbiol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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