1995 Fiscal Year Final Research Report Summary
Experimental evaluation of Japanese encephalitis vaccine candidates for human use generated by recombinant technology
| Project/Area Number |
06670328
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Kobe University |
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Principal Investigator |
KONISHI Eiji Kobe University School of Medicine Research Associate, 医学部, 助手 (40135786)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Japanese encephalitis / Vaccine / Antibody / T lymphocytes / Mouse / Immunity / recombinant virus |
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| Research Abstract |
Recombinant Japanese encephalitis (JE) vaccine candidates based on a highly attenuated vaccinia virus (NYVAC-JEV) and a canarypox virus (ALVAC-JEV) were evaluated for their ability to induce specific antibodies and cytotoxic T lymphocytes (CTLs) in mice. Six- to eight-week-old male Balb/c mice that received one or two intraperitoneal inoculations with these JE vaccine candidates at a dose of 1 * 10^7 PFU per mouse, produced neutralizing antibody and antibodies to the envelope (E) and non-structural 1 (NS1) proteins as determined by radioimmunoprecipitation. Immunization with either of these vaccine candidates also induced JE virus-specific T lymphocytes that proliferated in response to stimulation with infectious virus and/or non-infectious viral antigens. Mice maintained detedtable levels of neutralizing antibody and JE virus-specific memory T cells for at least 6 months after immunization with NYVAC-JEV and for 4 months after immunization with ALVAC-JEV.Cells induced to proliferate after stimulation with live virus contained specific CD8^+ CTLs that lysed primary Balb/c mouse kidney cells infected with JE virus and P815 mastocytoma cells infected with a recombinant vaccinia virus expressing the premembrane (prM), E,and NS1 proteins. These CTLs also lysed P815 cells infected with vaccinia recombinants expressing prM and E,and those expressing E and NS1, but did not lyse P815 cells infected with a recombinant virus expressing only NS1, indicating that the CTLs mainly recognized E,but did not recognize NS1. These results demonstrate that both recombinant JE vaccines, NYVAC-JEV and ALVAC-JEV,induce JE virus-specific antibody and CTLs in mice.
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