1995 Fiscal Year Final Research Report Summary
Structure and function of a novel human lectin P35
| Project/Area Number |
06670360
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Fukushima Medical College |
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Principal Investigator |
ENDO Yuichi Fukushima Medical College, Department of Biochemistry, Associate Professor, 医学部, 助教授 (20117427)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Animal lectin / Collagen-like domain / Fibrinogen-like domain / cDNA / Host defense / opsonin / gene structure |
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| Research Abstract |
Collectins are C-type lectins with collagenous and carbohydrate recognition domains and are involved in the first line host defense against pathogens. We report here a novel Ca^<2+>-dependent and ClcNAc-binding lectin consisting of subunit of 35 kDa (P35) with a collagenlike sequence. It forms a homopolymer by means of intermolecular disulfide bondingas is the case with collectins, P35 cDNA was cloned from a human liver cDNA library, and the deduced amino acid sequence of 313 residues revealed that the mature form of p35 consists mainly of collagen-and fibrinogen-like domains. The latter contained two protential Ca^<2+>-binding sites that may be involved in carbohydrate binding. The overall sequence of P35 was highly homologous to porcine ficolins alpha and beta. P35 enhanced phagocytosis of Salmonella typhimurium by neutrophils, suggesting an opsonic effect via the collagen region. Therefore, P35 represents a new type of GlcNAc-binding lectin with structural and functional similarities to collectins involved in innate immunity.
We isolated genomic clones of the P35 gene and found that P35 is encoded by eight exons. In addition, we isolated another distinct genomic clone corresponding to the upstream region of a P35-related gene which has an exon organization closely resembling that of the P35 gene. Northern blotting revealed that the P35 gene is expressed mainly in liver, whereas the P35-related gene is expressed in lung and peripheral blood leukocytes, demonstrating tissue-specific expression of these two genes. Both genes were assigned to a closely related region of chromosome 9 at 9934. Further studies are needed to elucidate the function of the P35-related gene product, and to establish the structural and functional relationship with P35.
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[Publications] Endo, Y., Onogi, S., Umeki, K., Yamamoto, I., Kotani, T., Ohtaki, S.and Fujita, T.: "Regional localization of the gene for thyroide peroxidase to human chromosome 2p25 and mouse chromosome 12C." Genomics. 25. 760-761 (1995)
Description
「研究成果報告書概要(和文)」より
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[Publications] Matsushita, M., Endo, Y., Taira, S., Sato, Y., Fujita, T., Ichikawa, N., Nakata, M. and Mizuochi,: "A novel human serum lectin with collagen-and fibrinogen-like domains which functions as an opsonin." J. Biol. Chem.271. 2448-2454 (1996)
Description
「研究成果報告書概要(和文)」より
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[Publications] Tamaki, H., Kaino, M., Endo, Y., Moriyama, T., Nakajima, T., Imagawa , Y.and Amino, N.: "Enzyme-linked immunosorbent assay for TSH receptor antibodies using the synthetic TSH receptor peptides." J.Clin.Lab.Immunol. 43. 89-98 (1994)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Endo, Y., Onogi, S., Umeki, K., Yamamoto, I., Kotani, T., Ohtaki, S.and Fujita, T.: "Regional localization of the gene for thyroide peroxidase to human chromosome 2p25 and mouse chromosome 12C." Genomics. 25. 760-761 (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ohishi, K., Inoue, N., Endo, Y., Fujita, T., Takeda, J.and Kinoshita, T.: "Structure and chromosomal localization of the GPI-anchor synthesis gene PIG-F and its Psuedogene psi PIG-F." Genomics. 29. 804-807 (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ohishi, K., Kurimoto, Y., Inoue, N., Endo, Y., Takeda, J.and Kinoshita, T.: "Cloning and characterization of th emurine GPI-anchor synthesis gene Pigf, a homologue of the PIGF gene." Genomics. (in press).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yoshimoto, T., Kojima, K., Funakoshi, T., Endo, Y., Fujita, T.and Nariuchi, H.: "Molecular cloning and characterization of murine IL-12 genes" J.Immunol. 156. 1082-1088 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Matsushita, M., Endo, Y., Taira, S., Sato, Y., Fujita, T., Ichikawa, N., Nakata, M.and Mizuochi, T.: "A novel human serum lectin with collagen-and fibrinogen-like domains which functions as an opsonin." J.Biol.Chem.271. 2448-2454 (1996)
Description
「研究成果報告書概要(欧文)」より
