1995 Fiscal Year Final Research Report Summary

BLOOD TYPING OF MIXED-STAIN COMSISTING OF THE SAME KIND OF BODY FLUIDS

Research Project

Project/Area Number	06670448
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Legal medicine
Research Institution	TOHOKU UNIVERSITY
Principal Investigator	SAGISAKA Kaoru Tohoku University, SCHOOL of MEDICAL FORENSIC MEDICINE,PROFESSOR, 医学部, 教授 (70006740)
Co-Investigator(Kenkyū-buntansha)	NATA Masayuki Tohoku University SCHOOL of MEDICAL FORENSIC MEDICINE,ASSISTANT, 医学部, 助手 (70241627)
Project Period (FY)	1994 – 1995
Keywords	MIXED STAINS / ABH BLOOD GROUP / ELISA
Research Abstract	FOR ABH BLOODTYPING MIXED-STAIN COMSISTING OF THE SAME KIND OF BODY FLUIDS,WE TRYED TO SPECIFIC ABSORBE A OR B BLOOD EPITOPE BY NITROCELLULOSE OR MICROTITE PLATE WHICH WAS COATED WITH ANTI-A OR-B ANTIBOY.HOWEVER,WE COULD NOT ABSORBE A OR B EPITOPE DUE TO LOW CAPACITY OF THE MEMBRANE AND PLATE.TO SEPARATE ABH EPITOPES OF MIXED STAIN SCOMSISTING OF DIFFERENT KIND OF BODY FLUIDS,WETTIED TO PREPARE MONOCLONAL ANTLIBODY AGAINST TEAR OR AMNITIC FLUID.IT WAS REVEALED THAT BOTH THE ANTIBOIES HAD SPECIFICITY TO PROTEINS IN TEAR OR AMNITIC FLUID carrying ABH blood group epitopes. IT IS CONSIDERED THAT THE MONOCLONAL ANTIBOIES COULD BE APLLYED TO FORENSIC PRACTICE TO DETECT SEPARATELY ABH BLOOD GROUP FROM MIXED STAINS CONSISTING OF DIFFERENT KIND OF BODY FLUID.

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