1995 Fiscal Year Final Research Report Summary
Bone Marrow Stromal cell-derived new growth factor for controlling the growth and apoptosis of myeloma cell
| Project/Area Number |
06670507
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内科学一般
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
SHIMIZU Shiro Kanazawa Medical University Associate Professor, 医学部, 助教授 (50097432)
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| Co-Investigator(Kenkyū-buntansha) |
MASAKI Yasufumi Kanazawa Medical University Assistant, 医学部, 助手 (40238895)
ARAI Toshihide Kanazawa Medical University Assistant, 医学部, 助手 (30193048)
TAKESHITA Shouichi Kanazawa Medical University Assistant, 医学部, 助手 (50247441)
FUKUTOKU Masaaki Kanazawa Medical University Assistant, 医学部, 助手 (20218944)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Multiple Myeloma / Growth Factor / Stroma Cell / Apoptosis |
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| Research Abstract |
From previous experiments, it was expected that an unknown bone marrow stromal cell derived myeloma cell growth factor playd an important role in the growth of myeloma cells. We have analyzed the growth factor activity to understand the mechanisms of myeloma cell growth.
Following data were obtained : 1) Three bone marrow stromal cell dependent myeloma cell lines, ILKM10, ILKM12 and ILKM13, were established. 2) All three cell lines grew absolutely dependently on bone marrow stromal cells. They did not proliferate in response to known cytokines including IL-6 or gp-130-related cytokines, but proliferate in response to bone marrow stromal cell culture supernatants. 3) The growth factor activity included in the culture supernatant was semipurified utilizing an ion exchange column chromatography and an anti-IL6 antiboy affinity column, and the myeloma growth factor activity was separated from IL-6. Data confirmed that at least another unknown factor playd an important role in the growth of myeloma cells.
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