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1995 Fiscal Year Final Research Report Summary

Specific interaction of pancreatic elastase and leukocytes to produce oxygen radicals and its implication in pancreatitis

Research Project

Project/Area Number 06670567
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionSapporo Medical University

Principal Investigator

WATANABE Naoki  Sapporo Nedical University, School of Medicine, Department of Laboratory Diagnosis, Professor, 医学部, 教授 (10158644)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Yasuo  Sapporo Medical University, School of Medicine, 4th Department of internal Medic, 医学部, 助手 (10236325)
Project Period (FY) 1994 – 1995
Keywordspancreatic elastase / oxygen radicals / neutrophil / specific binding site / NADPH oxidase / pancreatitis
Research Abstract

Many previous reports using experimental animal models of pancreatitis have suggested that oxygen free radicals plays an important role in initiation and development of pancreatitis. Infiltration of inflammatory cells, i.e., neutrophils, lymphocytes and monocytes, has been observed in damaged pancreatic glands of animal models and patients of pancreatitis. Neutrophils are known to be the highest producer of oxygen free radicals among these inflammatory cells, it seems plausible that oxygen free radicals produced by neutrophils have some pathoetiological meaning in pancreatitis. In the present study we measured the superoxide production by nutrophils obtained from patients with acute and chronic pancreatitis, and then examined the effects of pancreatic enzymes on superoxide production. Patients showed significantly higher superoxide production by 4beta-phorbol 12beta-myristate 13alpha-acetate (PMA)-stimulated neutrophils than healthy controls did. Among the three pancrestic enzymes, amylase, trypsin and elastase, elastase was the only one which increased the superoxide production by PMA-stimulated neutrophils, by an increment of 1.5-fold. It also increased the activity of NADPH oxidase prepared from PMA-stimulated neutrophils by a factor of 2.1. High affinity and low affinity binding sites fot elastase on neutrophils were identified. These results suggest that elastase plays a role in the development of pancreatitis by enhancing superoxide production of neutrophils.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Tsuji,N.,et al.: "Specific interaction of puncreatic elastase and leukocytes to produce oxygen randicals and its implication in panerakitis." Gut. 35. 1659-1664 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watanabe,N., et al.: "Cytotoxic effect of neutrophils-induced superoxide aguinst puncrenic aciner cells." Pancreas. 10. 415-417 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watanabe,N., et al.: "Endogenoustumor necrosis factor inhihit the cyloloxicity of exogenous tumor necrosis factor and adriamycin in puncreatic carcinoma cells." Pancreas. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuji, N., et al.: "Specific interaction of pancreatic elastase and leukocytes to produce oxygen radicals and its implication in pancreatitis" Gut. 35. 1659-1664 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, N., et al.: "Cytotoxic effect of neutrophilsinduced superoxide against pancreatic acinar cells" Pancreas. 10. 415-417 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, N., et al.: "Endogenous tumor necrosis factor inhibit the cytotoxicity of exogenous tumor necrosis factor and adriamycin in pancreatic carcinoma cells" Pancreas. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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