1995 Fiscal Year Final Research Report Summary
Purification and sequencing of intraluminal CCK-releasing peptide from rat.
| Project/Area Number |
06670582
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Showa University |
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Principal Investigator |
NAKANO Ikuta Showa University, School of Medicine, Assistant, 医学部, 助手 (20201668)
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| Co-Investigator(Kenkyū-buntansha) |
NOZU Fumihiko Showa University, School of Medicine, Assistant, 医学部, 助手 (30221485)
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| Project Period (FY) |
1994 – 1995
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| Keywords | pancreatic exocrine secretion / CCK-releasing peptide / STC-1 cell / bile |
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| Research Abstract |
Intraluminal protease inhibits pancreatic secretion and CCK release in conscious rat. To explain this phenomenon, it was hypothesized that the stimulation of pancreatic secretion and CCK release which occurs in the absence of luminal trypsin is caused by a trypsin-sensitive CCK-releasing peptide (CCK-RP). In this study, we purposed to investigate the mechanism of CCK release with purification and sequencing of the CCK-RP.Isolated rat intestinal epithelial cells and STC-1 cells (neuroendocrine tumors cells of transgenic mice) were tested for the ability of CCK release. CCK-RP was partially purified from the intestinal washes. The activity of CCK release was detected by using these cells. Partially purified CCK-RP was trypsin sensitive and the stimulation was calcium-dependent. Up to the present, the purification was not completed. But. in this study, we found that fats and bile also play an important role in CCK release. So, we examined the effect of bile on fat-stimulated pancreatic secretion in vivo. The results indicate that fat stimulates CCK release, and CCK release caused by fat was independent of intraluminal bile.
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