1995 Fiscal Year Final Research Report Summary
In vivo microscopic observation of neutrophil kinetics in pulmonary microcirculation.
| Project/Area Number |
06670603
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Chiba University |
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Principal Investigator |
KATOH Kunihiko Chiba University School of Medicine, Instructor, 医学部, 助手 (00204462)
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Osamu Chiba University School of Medicine, Instructor, 医学部, 助手 (60177045)
KURIYAMA Takayuki Chiba University School of Medicine, Professor, 医学部, 教授 (20009723)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Pulmonary microcirculation / In vivo microscopy / Ncutrophil / Pulmonary capillary / Pulmonary circulation / White blood ccll / Isolated perfused lobe |
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| Research Abstract |
The aims of this study were to establish a new method to evaluate neutrophil kiknetics in the pulmonary microcirculation and to clarify the mechanism for neutrophil passage through pulmonary circulation. Neutrophils, that were isolated from normal donor dogs, were labeled with fluorescein dye and injected into normal recipient dogs. And their passage through the pulmonary microcirculation was recorded by in vivo videofluorescence microscopy through a transparent thoracic window. During normal conditions, neutrophils were marginated in neither venules nor arterioles, but sequestered in the pulmonary capillaries. And also these neutrophils stopped exclusively at the junctions of the capillary network. This implies that the relationship between the size of neutrophil and the internal diameter of capillary segment has a significant role in neutrophil sequestration. These results suggests that the mechanism that causes ncutrophils to stop in the pulmonary microcirculation under normal conditions might be simple mechanical impediment.
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