• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1995 Fiscal Year Final Research Report Summary

Basic and clinical research of peripheral blood stem cell transplant (PBSCT) in chemotherapy for lung cancer

Research Project

Project/Area Number 06670615
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Respiratory organ internal medicine
Research InstitutionUniversity of Tokushima

Principal Investigator

SHIMIZU Eiji  University of Tokushima, School of Medicine Lecturer, 医学部, 講師 (50187449)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Akiyoshi  University of Tokushima, University Hospital of School of Medicine Senior reside, 医学部・附属病院, 医員
Project Period (FY) 1994 – 1995
KeywordsHigh dose chemotherapy / Lung cancer / Oncogene / NK cell / hematopoietic stem cell / PBSC / Peripheral blood stem cell transplant / RB / Cyclin / SCF
Research Abstract

Treatment of inoperable lung cancer consists of radiotherapy and chemotherapy, which have not improved the survival of the patients significantly because of myelosuppression including leukopenia. Peripheral blood stem cell transplant (PBSCT), an alternative of bone marrow transplant, which has recently developed in hematologic malignancy, may improve the survival of the inoperable lung cancer patients in combination with high dose chemotherapy. In this study, we examined the basic and clinical evaluation of PBSCT in patients with lung cancer. Cancer cell contamination was not found in any peripheral blood mononuclear cell sample from 15 patients. Survival rate and cytokine production of mononuclear cells stimulated by IL-12, ratio of CD34+ cell and CD33-/34+ cell, and number of CFU-GM in PBSC samples did not decrease after freezing and thawing. Under written informed consent, we did clinical application of PBSCT for two patients with small cell lung cancer and experienced rapid recovery from chemotherapy-induced myelosuppression. Megakaryocyte proliferation and differentiation induced by SCF closely related to increased expression of cyclin A and phosphorylation of RB tumor suppressor gene product. Natural killer (NK) activity of peripheral blood lymphocyte decreased after initiation of chemotherapy, reached to nadir 2 weeks later, and then recovered to pretreatment level 4-5 weeks later. Suppression of NK activity by high-dose chemotherapy was abrogated by PBSCT.These results suggest that PBSCT may contribute to not only hematopoietic recovery but also antitumor immunological recovery.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Shimizu E.et al.: "Differing effects of staurosporine and UCN-01 ON RB protein phosphorylation and expression of lung cancer cell lines." Oncology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimizu E et al.: "Stem cell factor regulates the expression of cyclin A and retinoblastoma gene product in the growth and differentiation pathway of human megakaryoblastic cells.J." Cancer Res.Clin.Oncol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimizu E et al.: "Patient with extensive small cell lung cancer given moderately doseintensified chemotherapy and peripheral blood stem cell support." Jpn.J.Clin.Oncol. 25. 212-217 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimizu,E.et al.: "RB protein status and clinical correlation from 172 cell lines representing lung cancer,extrapulmonary small cell carcinoma,and mesothelioma." Oncogene. 9. 2441-2448 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 清水英治: "肺小細胞癌に対する自家末梢血幹細胞移植術併用大量化学療法の検討-特に高齢患者よりの幹細胞採取条件について-" 日本外科系連合学会誌. 20. 185-190 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 清水英治他: "高齢者の肺癌" nanoGIGA. 3. 820-826 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 清水英治他: "肺小細胞癌におけるRB蛋白発現異常と肺非小細胞癌におけるサイクリンAの高発現" 小倉剛、安光勉編、中山書店、東京, 10 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 清水英治他: "発癌物質" 内科学書、島田馨編、中山書店、東京, 4 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 清水英治他: "癌遺伝子" 内科学書、島田馨編、中山書店、東京, 8 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 篠原明徳他: "抗癌剤の副作用と対策" 高杉益充編、医薬ジャーナル社、大阪, 11 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimizu E,et al.: "Differing effects of staurosporine and UCN-01 on RB protein phosphorylation and expression of lung cancer cell lines." Oncology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu E,et al.: "Stem cell factor regulates the expression of cyclin A and retinoblastoma gene product in the growth and differentiation pathway of human megakaryoblastic cells." J.Cancer Res.Clin.Oncol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu E,et al.: "Patient with extensive small cell lung cancer given moderately dose-intensified chemotherapy and peripheral blood stem cell support. Jpn." J.Clin.Oncol. 25. 212-217 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu E,et al.: "RB protein status and clinical correlation from 172 cell lines representing lung cancer, extrapulmonary small cell carcinoma, and mesothelioma" Oncogene. 9. 2441-2448 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1997-03-04  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi