1995 Fiscal Year Final Research Report Summary
Protease as the pathogenic factor of Pseudomonas cepacia pneumonia
| Project/Area Number |
06670617
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Kagawa Medical School |
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Principal Investigator |
FUJITA Jiro Kagawa Medical School Assistant Professor, 医学部, 助手 (80209056)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAJI Yasufumi Kagawa Medical School Assistant Professor, 医学部・附属病院, 助手 (00191297)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Pseudomonas cepacia / pneumonia / protease |
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| Research Abstract |
The present study was designed to examine the role of protease as a pathogenic determinant of P.cepacia. Clinically isolated strain of P.cepacia (KF-1), which was derived from a patient with P.cepacia pneumonia, and produced a significant amount of protease was used in this study. In comparison, the strain KFT1007, which was produced from KF-1 by the insertion of a single transposont gene, and lost the ability to release protease was also used. Immunosuppressed mice were challenged by intratracheal inoculation of 2*10^8 colony-forming units of P cepacia, and the survival rates were recorded. Between the strains which could produce protease and could not produce protease, there was an obvious difference in survival rates. In conclusion, our study suggests that protease plays an important role in the pathogenesis of P.cepacia.
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