1995 Fiscal Year Final Research Report Summary
Mucin Gene Expression and Airway Injury
| Project/Area Number |
06670636
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | University of Occupational and Environmental Health |
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Principal Investigator |
TSUDA Tohru University of Occupational and Environmental Health Dept.of Work Systems & Health, Associate Professor, 産業生態科学研究所, 助教授 (60207389)
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| Co-Investigator(Kenkyū-buntansha) |
MORIMOTO Yasuo University of Occupational and Environmental Health, Dept.of Environmental Healt, 産業生態科学研究所, 講師 (30258628)
OHMORI Hisamitsu University of Occupational and Environmental Health, Dev.of Respiratory Disease,, 医学部, 助手 (70271442)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Mucin Gene / Airway Secretion / MUC1 / MUC2 / Lung Cancer / Smoking / Asbestos / Adhesion Molecule |
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| Research Abstract |
We have undertaken a study to determine the expression of MUC2 in aiyway injury rat models. using RT-PCR.In Chrysotile and side stream of cigarette exposed Wistar rats' trachea, we could not find any MUC2 expression. In endotoxin exposed rats and mycoplasma infected rats, we could find mucin specific signals.
Polymorphic epithelial mucin (MUC1) has a transmembrane domain, which is simpler than that of gel-forming mucins (MUC2-7). There have been many studies in which mucin family genes were frequently expressed in malignant epithelial cells. These studies have suggested that they could play an important role in behavior of those cells. We have undertaken a study to determine the expression of MUC1, MUC2 and MUC5 in human lung cancer cell lines and specimens of resected lung cancer using RT-PCR technique. beta-actin was coamplified as an internal standard to quantitate PCR amplification of mRNA.
In 25 non-small cell lung cancer cell lines in which survival data were available, the Kapan-Meier survival curves demonstrated that those with high expression of MUC1+low expression of MUC2 survived for a shorter period of time (p=0.036).
In 59 resected non-small lung cancers, MUC1/beta-actin ratio was higher in non-small cell lung cancer than normal lung tissue (p=0.002). Moreover, the ratio of adenocarcinoma was higher than squamous cell carcinoma (p=0.052). There were weak tendencies between estimated surival rate and the MUC1/beta-actin rate.
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