1995 Fiscal Year Final Research Report Summary
Molecular biological changes of ADP/ATP carrier in cardiomypathic hamster
| Project/Area Number |
06670745
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
KATO Mitsutoshi Department of Internal Medicine, Auto Hospital, Jikei University School of Medicine, Assistant Prof., 医学部, 講師 (60177475)
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| Project Period (FY) |
1994 – 1995
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| Keywords | ADP / ATP carrier / J-2-N strain / Cardiomyopathic hamster |
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| Research Abstract |
ADP/ATP carrier protein (AAC) is an integral protein present in the inner mitochondrial membrane that performs the exchange of cytoplasmic and intramitochondrial ADP and ATP.AAC content of myocardium was studied in J-2-N cardiomyopathic hamsters. The AAC content was significantly decreased in the J-2-N hamsters. By molecular biological analysis, hamster AAC cDNA was cloned by the plaque hybridization method. The AAC cDNA hybridized specifically with AAC mRNA,so RNA dot-blot hybridization was performed. The highest AAC mRNA level was observed in control hamsters followed by J-2-N hamsters with mild myocardial damage, J-2-N hamsters with severe myocardial damage and Bio 14.6 cardiomyopathic hamsters. These results suggest that a decreased AAC content may contribute to the pathogenesis of cardiomyopathy and that a decrease of AAC mRNA levels may explain the abnormalities of AAC in J-2-N cardiomyopathic hamsters.
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