1995 Fiscal Year Final Research Report Summary
Effect of basic fibroblast growth factor on regional myocardial blood flow of acutely infarcted myocardium
| Project/Area Number |
06670751
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kinki University |
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Principal Investigator |
ISHIKAWA Kinji Dept Med, Kinki Univ, Professor, 医学部, 教授 (10088536)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAI Shoji Dept Med, Kinki Univ, Assistant, 医学部, 助手 (40268412)
KINO Hirofumi Dept Med, Kinki Univ, Assistant, 医学部, 助手 (10247976)
KOKA Hironari Dept Med, Kinki Univ, Assistant, 医学部, 講師 (50215208)
OGAWA Iwao Dept Med, Kinki Univ, Assistant, 医学部, 講師 (60204070)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Myocardial infarction / Collateral flow / regional myocardial flow / Basic fibroblast growth factor / Angiogenesis / Non radioactive colored microsphere |
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| Research Abstract |
This study was designed to clarify whether basic fibroblast growth factor (bFGF) might increase regional myocardial blood flow (Qm) at the acutely infarcted myocardium in experimental animals. Methods : In 22 SD rats, the chest was opened and the left coronary artery was ligated. The chest was closed and bred for 4 weeks. Nonradioactive colored microsphere technique was used to measure Qm. In 11 rats, bFGF was not used while in the other 11 rats, bFGF 100mug was injected into the infarcted myocardium immediately after coronary occlusion. After 4 weeks, the heart was excised for the histological examinations. In 25 dogs, the left anterior descending coronary artery was ligated and bred for 4 weeks. Qm was measured using nonradioactive colored microsphere technique. In 11 dogs, bFGF 300mug was injected into the myocardium just before coronary occlusion, while in the other 14 dogs, bFGF was not given. Results : In rats, bFGF failed to change Qm of the infarcted myocardium as well as border zone and noninfarcted myocardium. Infarct size was not reduced in rats by bFGF.In dogs, Qm in control group and bFGF group were 24(]SY.+-.])4,63(]SY.+-.])10% respectively at the inner layr ; 23(]SY.+-.])5,100(]SY.+-.])20% at the middle layr and 74(]SY.+-.])7,130(]SY.+-.])16% at the outer layr. This study showed thatr bFGF increased Qm at the infarcted myocardium in dogs while bFGF had no effect in rats.
Conclusion : Basic FGF had a significant effect to increase Qm at the infarcted myocardium in dogs. This may offer a new method for the treament of ischemic heart disease.
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