1995 Fiscal Year Final Research Report Summary
Aberration of TAL1 gene is specifically involved in childhood T-cell leukemia and its functional analysis
| Project/Area Number |
06670772
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | University of Tokyo |
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Principal Investigator |
KOBAYASHI Shigetoshi Univ.of Tokyo(Hospital)Pediatrics, Assistant Professor, 医学部(病), 助手 (70260487)
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| Co-Investigator(Kenkyū-buntansha) |
BESSHO Fumio Univ.of Tokyo(Hospital)Pediatrics, Associate Professor, 医学部(病), 助教授 (40010285)
HAYASHI Yasuhide Univ.of Tokyo(Hospital)Pediatrics, Associate Professor, 医学部(病), 講師 (30238133)
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| Project Period (FY) |
1994 – 1995
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| Keywords | TAL1 gene / acute lymphoblastic leukemia / T-cell malignancies / minimal residual disease / PCR |
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| Research Abstract |
Site-specific recombination of the TAL1 gene was analyzed by Southern blotting and polymerase chain reaction (PCR) in 44 cases of childhood T-cell acute lymphoblastic leukemia (T-ALL), 20 cases of childhood T-cell non-Hodgkin's lymphoma (T-NHL) and 35 cases of adult T-cell malignancies.This recombination was found in 10 (22.7%) of 44 childhood T-ALL patients, but in none of the T-NHL or adult T-cell malignancies.Recombination of the TAL1 gene was therefore suggested to be specific for childhood T-ALL.The immunophenotypic features of the 10 T-ALL patients with this recombination were CD1-, CD2+, CD4-, CD7+, CD10-, and they had a significantly better outcome than other T-ALL cases without the recombination.The PCR technique revealed minimal residual disease (MRD) in 4 patients.One showed persistent MRD,while in the other MRD was recognized only at initial diagnosis. Further investigation is needed whether T-ALL with this recombination constitutes a distinct clinical subgroup among childhood T-ALL patients.
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Research Products
(12 results)
