1995 Fiscal Year Final Research Report Summary
Studies on the expressin of adhesion molecules on Fibroblats and lenhaytes by cytonagslovirus
| Project/Area Number |
06670784
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Mie University |
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Principal Investigator |
ITO Masahiro Mie University School of Midicin, Department of Pediatrics Associate Professor, 医学部, 助教授 (10126956)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Cytomegalovirus / Adhesion moleacles / Cytotoxicity |
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| Research Abstract |
We investigated the adherence of peripheral blood leukocytes to cytomegalovirus (CMV) -infected and uninfected fibroblasts. Adherence of fresh peripheral blood mononuclear cells (PBMC) to CMV-infected fibroblasts was significantly greater than their adherence to uninfected fibroblasts. In leukocyte populations, fresh monocytes and B cells adhered to CMV-infected fibroblasts, whereas fresh T cells and polymorphonuclear leukocytes (PMNL) did not adhere to either CMV-infected or uninfected fibroblasts. When T cells were activated with anti-CD3 antibody, activated T cells demonstrated the adherence and cytotoxicity to both CMV-infected and uninfected fibroblasts. Magnitude of cytotoxicity mediated by adherent activated T lymphocytes was significantly greater that that of unifected fibroblasts. Adherence of PBMC and cytotoxicity mediated by adherent activated Tcells were blocked by treatment of CMV-infected fibroblasts with anti-ICAM-1 antibody and by treatment of leukocytes with anti-LFA-1 antibody. These data suggest that an interaction of ICAM-1 and LFA-1 is responsible for the adherence oc leukocytes and for adherent activated T cell-mediated cytotoxicity against CMV-infected fibroblasts.
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