1995 Fiscal Year Final Research Report Summary
A study of low birth weight infant and uniparetal disomy
| Project/Area Number |
06670799
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Yamaguchi University |
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Principal Investigator |
TSUKAHARA Masato Yamaguchi University School of Medicine Department of Pediatrics, Associate professor, 医学部, 助教授 (20136188)
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| Co-Investigator(Kenkyū-buntansha) |
TATEISHI Hiroshi Yamaguchi University School of Medicine Department of Pediatrics, Assistant prof, 医学部・附属病院, 助手 (00253154)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Low birth weight infant / Uniparental disomy / Chromosomal abnormality / PCR |
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| Research Abstract |
1.A total of 7 low birth weight infants (small-for-dates : SFD) were evaluated for chromosomal abnormalities and uniparental disomy. Blood samples from 7 babies and their parents were obtained after informed consent. Chromosome analysis and polymerase chain reaction (PCR) using oligonucleotides were performed. PCR proceeded with oligonucleotides showing repeat sequences from each chromosome as primers and genomic DNA as a template. Neither chromosomal abnormalities nor uniparental disomy was demonstrated in each baby.
2.A total of 12 spontaneous abortuses were also evaluated for the presence of chromosomal abnormalities and uniparental disomy. Of these 12 abortuses, one revealed abnormal karyotype 46, XX,-4, t (4 ; 7) (q35 ; p13) which was derived from paternal balanced translocation 46, XY,t (4 ; 7) (q35 ; p13). No uniparental disomy was detected in each abortus.
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