• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1995 Fiscal Year Final Research Report Summary

Cytokine gene expression in atopic dermatits

Research Project

Project/Area Number 06670870
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionNagasaki University

Principal Investigator

TANAKA Yoichi  Nagasaki University, School of Medicine, Lecturer, 医学部, 講師 (20231417)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Kenshi  Nagasaki University, School of Medicine, Assistant, 医学部, 助手 (90240093)
Project Period (FY) 1994 – 1995
Keywordsatopic dermatitis / Th1 / Th2 / IL-4 / IFN-gamma / in situ hybridization
Research Abstract

To date, a number of findings suggesting a predominance of type 2 helper T cell (Th2) have been reported in atopic dermatitis (AD). On the other hand, a predominance of interferon-gamma (IFN-gamma) messenger RNA (mRNA) expression was recently demonstrated by reverse transcription polymerase chain reaction (RT-PCR) in the lesions of AD.To address the controversial issue, we investigated both transcription and translation of interleukin-4 (IL-4) and IFN-gamma in the lesions of five AD patients by in situ hybridization (ISH) and immunostaining. In mRNA and protein level, coexpression of IL-4 and IFN-gamma was observed. The number of IFN-gamma mRNA-positive cells (14.0<plus-minus>6.7) was significantly higher than that of IL-4 mRNA-positive cells (2.4<plus-minus>1.1) (p=0.04). The number of positive staining cells of IFN-gamma (6.3<plus-minus>2.8) were also significantly higher than that of IL-4 (1.8<plus-minus>1.2) (p=0.04). However, the number of IL-4 mRNA-positive cells tended to correlate with the AD severity (r=0.76, p=0.13), suggesting an important role of IL-4 in the pathogenesi

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] Tanaka Y et al: "Coexpression of interleukin-4 and interferon-γ messenger RNA in lesional skin of atopic dermatitis" (in preparation).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanaka Y,Zhang HM,Bae SJ,Hakugawa J,Takenaka M,Anan S and Katayama I: "Coexpression of interleukin-4 and interferon-gamma messenger RNA in lesional skin of atopic dermatitis" (in preparation).

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1997-03-04  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi