1995 Fiscal Year Final Research Report Summary
Cytokine gene expression in atopic dermatits
| Project/Area Number |
06670870
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Nagasaki University |
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Principal Investigator |
TANAKA Yoichi Nagasaki University, School of Medicine, Lecturer, 医学部, 講師 (20231417)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Kenshi Nagasaki University, School of Medicine, Assistant, 医学部, 助手 (90240093)
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| Project Period (FY) |
1994 – 1995
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| Keywords | atopic dermatitis / Th1 / Th2 / IL-4 / IFN-gamma / in situ hybridization |
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| Research Abstract |
To date, a number of findings suggesting a predominance of type 2 helper T cell (Th2) have been reported in atopic dermatitis (AD). On the other hand, a predominance of interferon-gamma (IFN-gamma) messenger RNA (mRNA) expression was recently demonstrated by reverse transcription polymerase chain reaction (RT-PCR) in the lesions of AD.To address the controversial issue, we investigated both transcription and translation of interleukin-4 (IL-4) and IFN-gamma in the lesions of five AD patients by in situ hybridization (ISH) and immunostaining. In mRNA and protein level, coexpression of IL-4 and IFN-gamma was observed. The number of IFN-gamma mRNA-positive cells (14.0<plus-minus>6.7) was significantly higher than that of IL-4 mRNA-positive cells (2.4<plus-minus>1.1) (p=0.04). The number of positive staining cells of IFN-gamma (6.3<plus-minus>2.8) were also significantly higher than that of IL-4 (1.8<plus-minus>1.2) (p=0.04). However, the number of IL-4 mRNA-positive cells tended to correlate with the AD severity (r=0.76, p=0.13), suggesting an important role of IL-4 in the pathogenesi
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