1995 Fiscal Year Final Research Report Summary
Molecular biology of morphological preservation of radiotherapy
| Project/Area Number |
06670894
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
SHIRATO Hiroki Hokkaido Univ., Fac. of. Med.Assi. Pro, 医学部附属病院, 講師 (20187537)
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| Co-Investigator(Kenkyū-buntansha) |
YASUDA Motoaki Hokkaido Univ., Fac. of Dent. Instructor, 歯学部, 助手 (90239765)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Radiation / Cell adhesion / Morphorogic preservation / Cadherin Molecule |
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| Research Abstract |
To investigate the mechanism of morphological preservation of radiation therapy (RT), clinical, pathological, and molecular biological research was undertaken. By photographical and radilogical comparison between the pre-RT morphology and post-RT morphology, it was conclusive that morphological preservation is not merely ordinal tissue repair, but rather complex regeneration. Immunostaning of E-cadherin, which is one of the cell-cell adhesion molecule important in morphological developmement, showed that E-cadherin is stained even 5 years after the 65 Gy irradiation to the larynxgeal surface. Cell migration assay showed that more number of epitherial cells, HSC3 and CA9-22, were migrated by the conditioned medium of MRC5 which was irradiated 10 Gy than that of non-irradiated MRC5 Immunobiochemical quantitative analysis showed that the conditioned medium of MRC5 has more amount of HGF after 10 Gy irradiation than that of non-irradiated MRC5. These resultus suggested that morphological preservation after radiotherapy is depend on the molucular control of normal tissue, of which mechanism still await more investigation.
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