1996 Fiscal Year Final Research Report Summary
Basic research for glucose metabolism in inflammatory tissue by positron-labelled glucose analog
| Project/Area Number |
06670899
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Tohoku University |
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Principal Investigator |
YAMADA Susumu Institute for Development, Aging & Cancer, Department of Nuclear Medicine & Radiology, Tohoku University Assistant Professor, 加齢医学研究所, 講師 (70182532)
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| Co-Investigator(Kenkyū-buntansha) |
KUBOTA Kazuo Institute for Development, Aging & Cancer, Department of Nuclear Medicine & Radi, 加齢医学研究所, 助教授 (40161674)
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| Project Period (FY) |
1994 – 1996
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| Keywords | glucose metabolism / FDG / inflammation / autoradiography / tissue distribution |
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| Research Abstract |
1.Tissue distribution of ^<18>FDG in turpentine-induced inflammatory tissue was investigated. Maximum uptake of ^<18>FDG was observed 1 hour after the ^8FDG injection and the uptake in chronic phase was higher than that in acute phase.
2.Relations between the inflammatory composition factor and the ^<18>FDG uptake was examined by macro and micro-autoradiography (ARG). On macro-and micro-ARG,the highest grain density due to ^<18>FDG uptake was observed in marginal zone of abscess wall consisting many inflammatory cells of neutrophils and macrophages, epithelial cells of blood vessel and young fibroblasts. Results indicate that increased permeability of capillary blood vessels result in a leakage of fluid including ^<18>FDG into the extra-vascular space and the ^<18>FDG was utilized as energy source. Relations between other factors of vascular permeability and blood glucose and ^<18>FDG uptake were also examined. Double-tracer tissue distribution study, of ^<131>I serum albumin as indicator of vascular permeability and the ^<18>FDG showed that the ^<18>FDG uptake depended on the vascular permeability. In glucose and insulin loaded tissue distribution study, ^<18>FDG uptake in inflammatory tissue in control group was lower than in glucose loaded group and insulin group, thus indicating the complicated influence of blood glucose and insulin on glucose metabolism in inflammtory tissue.
3.In granulocyte-colony-stimulating-factor (G-CSF) loaded tissue distribution study, ^<18>FDG uptakes in inflammatory tissue, spleen and bone were significantly higher than those in control group. This result may reflects the direct function of G-CSF as cytokine.
4.In double-tracer tissue distribution study of ^<67>Ga citrate and ^<18>FDG,both maximum uptakes were also observed on Day 4 after inoculation of turpentine-oil. However, on double-tracer macro-ARG,the highest uptake of ^<67>Ga citrate was observed outside abscess wall and the highest of ^<18>FDG was inside.
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