1995 Fiscal Year Final Research Report Summary
Effects of CSF from schisophrenic patients on growth and differentiation of neurobl a stoma cells
| Project/Area Number |
06670953
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Psychiatric science
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| Research Institution | Chiba university |
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Principal Investigator |
SHIRASAWA Hiroshi Chiba Univ. School of Medicine, Assistant, 医学部, 助手 (00216194)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAMOTO Tadashi Chiba Univ. School of Medicine, Assistant, 医学部, 助手 (10221269)
KODAMA Kazuhiro Chiba Univ. School of Medicine, Lecturer, 医学部, 講師 (90161942)
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| Project Period (FY) |
1994 – 1995
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| Keywords | schizophrenia / neural cell / differentiation |
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| Research Abstract |
Effects of CSF from schizophrenic patients on a neuroblastoma cell line RT-BM-1 were studied. The CSFs taken from two informed-consent schizophrenic patients were added to the culture medium. The growth rate, population doubling time and saturation density of RT-BM-1 Cultared in the CSF-added medium were sameas those of control cells. Effects of CSFs from schizophremic patients on the differentiation of RT-BM-1 cells by retinoic acid were also examined. However, no differences were observed in the morphological changes induced by the retinoic acid as well as the amount of MAP 2 demonstrated by western blotting.
To investigate further the etiology of the schizophrenia, we attempted to clone cDNAs of genes differentially expressed by adminstration of Phencylidine (PCP) . We have identified several unknown and known genes differentially expressed genes in brains of PCP-administrated rats. We also cloned the cDNA of the mouse mxil gene (E.Shimizu et al., 1995) , and found that the expression of the mxil elevates during neuronal differntiation (E.Shimizu et al., in press).
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[Publications] Simizu, E., Sirasawa, H., Kodama, K., Koseki, K., Sato, T., and Simizu, B.: "Cloning and sequencing of the murine Mxil cDNA." Gene. 152. 283-284 (1995)
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