1995 Fiscal Year Final Research Report Summary
PATHOPHYSIOLOGICAL ROLE OF SCP2 IN ATHEROSCLEROSIS
| Project/Area Number |
06671003
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
内分泌・代謝学
|
|---|
| Research Institution | CHIBA UNIVERSITY |
|---|
Principal Investigator |
HIRAI Aizan CHIBA UNIVERSITY MEDICAL SCHOOL HOSPITAL ALECTURER, 医学部付属病院, 講師 (10189813)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TERANO Takashi CHIBA UNIVERSITY MEDICAL SCHOOL HOSPLTAL ASSISTANT, 医学部付属病院, 助手 (70237006)
|
|---|
| Project Period (FY) |
1994 – 1995
|
| Keywords | ATHEROSCLEROSIS / MACROPHAGE / MODIFEID LDL / SCAVENGER RECEPTOR / LIPID TRANSFER PROTEIN / SCP2 / CHOLESTEROL / FOAM CELL FORMATION |
|---|
| Research Abstract |
Sterol carrier protein 2 (SCP_2) has been shown to be involved in intracellular transport and metabolism of cholesterol. However, there have been no reports concerning SCP_2 in macrophages, the major source of atheromatous foam cells. We investigated whether SCP_2 is present in rat peritoneal macrophages and determined the changes of SCP_2 and its mRNA levels in macrophages during foam cell formation induced by acetylated LDL(AcLDL).
Immunoblot analysis and Northern blot analysis demonstrated that both SCP_2 and its mRNA are expressed in rat peritoneal macrophages. Incubations with AcLDL caused a dose-and time-dependent increase of cellular esterified cholesterol, SCP_2 and its mRNA in rat peritoneal macrophages. The inhibitor of acyl-CoA : cholesterol acyltransferase further enhanced AcLDL-induced increase of SCP_2 protein and its mRNA.Incubations with 25-hydroxy cholesterol also caused a dose-dependent stimulation of SCP_2 gene expression in macrophages, while incubation with maleylated BSA had no effect. These results suggest that the increment of cellular free cholesterol is responsible for enhanced SCP_2 gene expression in macrophages. The enhancement of SCP_2 gene expression by AcLDL suggests that SCP_2 may play an important role during foam cell formation induced by AcLDL which may be most important step for the atherosclerosis.
|
-
-
-
-
-
-
-
[Publications] Hirai, A., Kino, T., Tokinaga, K., Tahara, K., Tamura Y., Yoshida, S.and Suzuki, K.: "Regulation of gene expression sterol carrier protein 2 (SCP2) during foam cell formation of macrophage" J Lipid Bicohem. 36(in japanese). 255-257 (1994)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Yoshida, S., Hirai A., Kino, T., Tokinaga, k., tahara, K., Tamura, Y.: "Presence and the regulation of gene expression of Sterol carrier protein2 (SCP2) in FRTL-5 cells" ibid. 37(in japanease). 258-260 (1995)
Description
「研究成果報告書概要(欧文)」より
