1995 Fiscal Year Final Research Report Summary
Purification and characterization of LDL containing apo B-48 from the plasma of an apo E-deficient patient
| Project/Area Number |
06671006
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
内分泌・代謝学
|
|---|
| Research Institution | University of Tokyo |
|---|
Principal Investigator |
HASHIMOTO Yoshiaki University of Tokyo, First Dept.of Int.Med.Assistant Prof., 医学部, 講師 (40172879)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
WATANABE Tsuyoshi University of Tokyo, First Dept.of Int.Med.Associate Prof., 医学部, 助教授 (80158641)
HORIE Yukio University of Tokyo, First Dept.of Int.Med. Prof., 医学部, 医員 (40211552)
|
|---|
| Project Period (FY) |
1994 – 1995
|
| Keywords | apo B-48 / apo B-100 / apo E / chyromicron metabolism / apo A-I / apo A-IV |
|---|
| Research Abstract |
The plasma low density lipoprotein (LDL) of an apoprotein (apo) E-deficient patient was reported to contain apo B-48 (D.Kurosaka et al. (1991) Atheroscler.88,15), which is a structural protein of chylomicrons and is not present in the fasting plasma LDL of normal subjects. We separated the LDL containing apo B-48 (apo B-48 LDL) and apo B-100 (apo B-100 LDL) from the plasma of this patient using heparin-Sepharose column chromatography. The apo B-48 LDL contained apo A-I,apo A-IV,and apo Cs besides apo B-48. There were only slight differences in size and lipid composition of the apo B-48 and the apo B-100 LDLs, both of which contained more triglyceride than LDL from normal subjects. After incubation with apo E,the apo B-48 LDL contained twice the amount of apo E than the apo B-100 LDL and showed a marked decrease in apo A-I and apo A-IV content. These results suggest that lipoproteins containing apo B-48 in normal subjects can be quickly taken up through an apo E-mediated receptor mechanism after receiving apo E in exchange for apo A-I and A-IV.
|
