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1995 Fiscal Year Final Research Report Summary

Functional Characteristics in the Embryo of Mouse Model of Transposition of the Great Arteries

Research Project

Project/Area Number 06671172
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Embryonic/Neonatal medicine
Research InstitutionTokyo Women's Medical College

Principal Investigator

NAKAZAWA Makoto  Tokyo Women's Medical College, Dept of Pediatr Cardiol, Professor, 医学部, 教授 (10075567)

Co-Investigator(Kenkyū-buntansha) MORISHIMA Masae  Tokyo Women's Medical College, Research Division, Assistant, 医学部, 助手 (00241068)
YASUI Hiroshi  Tokyo Women's Medical College, Dept of Develop Biology, Assistant, 医学部, 助手 (60210241)
TOMITA Sachiko  Tokyo Women's Medical College, Dept of Pediatr Cardiol, Assistant, 医学部, 助手 (40231451)
Project Period (FY) 1994 – 1995
KeywordsCardiac Anomaly / Mouse Embryo / Retinoic Acid / Transposition of the Great Arteries / Hemodynamics
Research Abstract

We had been trying to make a good model of transposition of the great arteries (TGA) in the mouse by treating pregnant dams by all-trans retinoic acid, and finally established the model in which TGA was induced in 90% of embryos by giving the drug with a dose of 70mg/kg at 8.5 days of gestation. Using this model, we have found that hypo-dysplasia of proximal outflow swelling causes failure of the normal ventriculo-arterial ralation and leads to parallel connection of the ventricles to the great arteries, i.e.the right ventricle (RV) to the aorta (Ao) and the left ventricle (LV) to the pulmonary artery (PA). We have then considered that it is important to find the role of hemodynamics in its morphogenesis, and established the technique in which the mammalian embryo is observed in situ under a microscope and action of the heart is recorded with a high speed video camera while it is still alive and viable. Using this method, it was found that there was two pattaerns of flow through outflow tract of the primitive ventricle in TGA model embryos ; one is that flow from RV to Ao was parallel to and separated from it from LV to PA ; the other was that flow from RV goes into both Ao and PA.The latter likely destined to TGA associated with malalign type ventricular septal defect, or double outlet right ventricle in an extreme case. Analysing the ventricular size and function, RV was smaller and lower in ejection fraction in TGA embryos than in controls, suggestings some possible role of hemodynamics in pathogenesis of TGA.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Nakazawa M,et al: "Hemodynamics and ventricular function in the day-12 rat embryo;basic characteristics and the responses to cardiovascular drugs." Pediatric Research. 37. 117-123 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasui H,et al: "Morphological observation on the pathogenetic process of transposition of the great arteries induced bu retinoic acid in mice." Circulation. 91. 2478-2486 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morishima M,et al: "Visceroatrial heterotaxy syndrome induced by maternal hyperthermia in the rat." Cardiol Young. 5. 251-256 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 宮川・富田幸子: "心臓の形態発生とその異常" AD&S. 1. 11-15 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyagawa-Tomita S,et al: "Pathological study of Japanese quail embryo with acid alpha glucosidase defeciency early development." Acta Neuropathol. (in print).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasui H,et al: "The cardiac outflow tract septation in the mouse model of transposition of the great arteries" Circulation. (投稿中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakazawa M et al:"Developmental Mechanisms of Heart Disease" Clark EB,Markwald RR,Takao A,(ed),Futura Publ,Mount Kisco,NY,679 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 富田幸子、中澤誠: "先天性心疾患・小児の心疾患" 中澤誠編集、循環器NOW,南江堂, 319 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakazawa M,Morishima M,Tomita H,Miyagawa-Tomita S,Kajio F: "Hemodynamics and ventricular function in the day-12 rat embryo ; basic characteristics and the responses to cardiovascular drugs." Pediatric Reseaerch. 37. 117-123 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasui H,Nakazawa M,Morishima M,Miyagawa-Tomita S,Momma K: "Morphological observation on the pathogenetic process of transposition of the great arteries induced bu retinoic acid in mice." Circulation. 91. 2478-2486 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morishima M,Miyagawa-Tomita S,Yasui H,Ando M,Nakazawa M,Takao A: "Visceroatrial heterotaxy syndrome induced by maternal hyperthermia in the rat." Cardiol Young. 5. 251-256 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyagawa-Tomita: "SAFCardiac development and Abnormal morphogenesis." AD&S (in Japanese). 1. 11-15 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyagawa-Tomita S,Morishima M,Nakazawa M,et al: "Pathological study of Japanese quail embryo with acid alpha glucosidase defeciency early development." Acta Neuropathol. (in print).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasui H,Morishima M,Ando M,Takao A,Aikawa E,Nakazawa M: "The cardiac outflow tract septation in the mouse model of transposition of the great arteries." Circulation. (in submission).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakazawa M et al: Functional characteristic of the embryonic heart. in Clark EB,Markwald RP,and Takao A (ed) Development Mechanisms of Heart Diseases.Futura Publ, Mount Kisco, NY, 435 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyagawa-Tomita S,Nakazawa M: On the mechanisms of morphogenesis of cardio vascular anomalies. In Nakazawa M (ed) Congenital and Aquired Heart Diseases in Childhood. Nankoudo, Tokyo, (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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