1996 Fiscal Year Final Research Report Summary
Role of nitric oxide in septic shock
| Project/Area Number |
06671199
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Osaka University |
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Principal Investigator |
TANAKA Hiroshi Osaka University Medical School Assistant Professor, 医学部, 助手 (90252676)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMAZU Takeshi Osaka University Medical School Lectufer, 医学部, 講師 (50196474)
YOSHIOKA Toshiharu Osaka University Medical School Associate Professor, 医学部, 助教授 (60127313)
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| Project Period (FY) |
1994 – 1996
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| Keywords | nitric oxide (NO) / sepsis / cytokine / ischemia reperfusion injury |
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| Research Abstract |
This study is designed to investigate the role of nitric oxide (NO) and other chemical mediators in septic shock and ischemia-reperfusion injury.
1)Serum cytokine (TNF,IL-6,8 and G-CSF) and NO_2-/NO_3- concentration (NOx) were measured serially in 15 sepsis patients. Changes in those levels were compared with those in neutrophil phagocytic and bactericidal activities. Serum cytokine as well as NOx levels were markedly elevazted at diagnosis and remained high during the course. These levels decreased significantly in the 11 survivors. Neutrophil function remained normal during the course of disease. These results suggest that G-CSF as well as other inflammatory cytokines may play an important role in the maturation and maintenance of function of neutrophils during the inflammatory response of sepsis.
2)The aim of this experiment study was to clarify the profile of topical NO synthesis caused by transient global ischemia in Mongolian gerbils. The NOx level in the dorsolateral portion of the striatum measured by the microdialysis decreased during ischemia and elevated following reperfusion. The elevation was inhibited with L-NMA.Those results suggest that NOx plays an important role in the regulation of ischemia-reperfusion injury in brain.
3)To clarify the role of leukocyte in reperfusion injury, we measured the resting transmembrane potential difference (Em) and evaluated postcapillary venule microcirculation in rat skeletal muscle during ischemia and reperfusion. Persistent depolarization in the Em was observed in an ischemia-reperfusion group. The adherent leukocyte count significantly increased following reperfusion. These results suggested that leukocyte plays an important role in tissue injury following ischemia-reperfusion.
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