1995 Fiscal Year Final Research Report Summary
Growth inhibition of human cancer cells in vivo by antisense c-myc phosphorothioate oligonucleotide
| Project/Area Number |
06671213
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
NAKASHIMA Hideaki MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY,LECTURER, 生体防御医学研究所, 助手 (20253528)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBUTA Henji MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY,LECTURER, 生体防御医学研究所, 助手 (70253531)
MURAKAMI Akira KYOTO INSTITUTE OF TECHNOLOGY,DEPARTMENT OF POLYMER SCIENCE AND ENJINEERING PROF, 繊維学部, 教授 (60210001)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Antisense oligonucleotide / c-myc / Breast cancer / in vivo effects / Prothymosin-alpha |
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| Research Abstract |
In order to determine the role of c-myc in the growth of human breast cancer cells, we examined the in vivo effects of a synthetic antisense c-myc phosphorothioate oligonucleotide on the heterotransplanted human breast cancer cell line, MCF-7, in nude mice. Pathohistologically, tumors treated with intratumorous injection of antisense c-myc phosphorothioate oligonucleotide revealed massive necrosis, suggesting the antitumor effects of antisense c-myc oligonucleotide. As the second step, a semi-quantitative assay for measuring c-myc mRNA expression by utilizing a RT-PCR method and in vitro assay for assessing the growth inhibitory effects of antisense c-myc oligonucleotide on the primary culture cells were deviced. The data suggeted that the growth inhibition by antisense c-myc oligonucleotide was more evident in the tumors with higher leves of the c-myc mRNA expression than those with low leves of the c-myc mRNA expression. As the third step, in order to accurately determine the role of prothymosin, a new prognostic factor in gastric cancer, the relationship between the prothymosin mRNA expression and the c-myc mRNA expression was examined. Since the mRNA expression levels of these genes showed a significant cerrelation, an application of antisense c-myc phosphorothioate oligonucleotide to cancer cells should provide an useful information for the interrection between prothymosin-alpha and c-myc.
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