1995 Fiscal Year Final Research Report Summary
Analysis of gene expression of matrix metalloproteinases cluring the metastasis of gastrointestinal tract cancer
| Project/Area Number |
06671227
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Keio University |
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Principal Investigator |
OTANI Yoshihide Keio Univ.School of Med, Dept of Surgeny, 医学部・外科学教室, 助手 (20168983)
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| Co-Investigator(Kenkyū-buntansha) |
SAKURAI Yoshiko Keio Univ, School of Med., Dept of Surgeny, 医学部・外科学教室, 助手 (90245514)
KAMEYAMA Kaori Keio Univ, School of Med., Dept of Pathology, 医学部・病理学教室, 助手 (10245467)
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| Project Period (FY) |
1994 – 1995
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| Keywords | GI tract cancer / metastasis / MMP / collagenase / mesenchymal cell / extracellular matrix / collagen / in situ hybridization |
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| Research Abstract |
During cancer invasion and metastasis, anscer cells must degradate extracellular matrix around cancer nests. We have already reported that proteolytic enzyme activity correlated to the degree of malignancy of the cancer (Keio J.Med.39 : 159-167,1990).
In this study, we tried to analize which cells produce these enzymes (matrix metalloproteinases : MMPs) and how they are regulated in the local region. For this purpose, in situ hybridization using cDNA probe for MMP-1 was carried out in GI tract cancer tissues.
We have recealed that stromal cells and blood cells, especially eosinophiles, produce MMP-1 and could play impotant roles in cancer invasion and metastasis.
In conclusion, not only cancer cells but host cells such as fibroblasts and eosinophiles are regulating the steps of degradation of extracellular matrix during cancer invasion and metastasis.
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