1996 Fiscal Year Final Research Report Summary
The role of adhesion molecule between mesothel and cancer cells and anti-adhesion therapy against peritoneal metastasis.
| Project/Area Number |
06671241
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Gunma University School of Medicine |
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Principal Investigator |
ASAO Takayuki GUNMA UNIVERSITY,SURGERYI,School of Medicine ASSISTANT, 医学部, 助手 (40212469)
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| Co-Investigator(Kenkyū-buntansha) |
YAZAWA Shin GUNMA UNIVERSITY LEGAL MEDICINE,School of Medicine ASOSIATED PROFESSOR, 医学部, 講師 (10008386)
SITARA Yosinori GUNMA UNIVERSITY SURGERYI,School of Medicine ASSISTANT, 医学部, 助手 (80251114)
NAGAMACHI Yukio GUNMA UNIVERSITY SURGERYI,School of Medicine PROFESSOR, 医学部, 教授 (30008289)
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| Project Period (FY) |
1994 – 1996
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| Keywords | Metastasis / Cancer cell / Cell adhesion / Adhesion molecule / Peritoneal metastasis |
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| Research Abstract |
Adhesion molecules associating with peritoneal dissemination were investigated using human gastric (MKN45 and MKN74) and colon (KMI2C and KMI2SM) cancer cells and the mouse peritoneum. Adhesion of cancer cells to the peritoneum was determined by a recently reported novel ex vivo method. MKN45 cells established from poorly differentiated adenocarcinoma with less glycosylated sugar chains on their cell surface showed higher adhesion activities to the peritoneum ex vivo and produced large amount of metastases in the abdominal cavity of nude mice, whereas MKN74 cells from differentiated adenocarcinoma with more glycosylated sugar chains showed slightly low adhesion activity. KMI2SM cells with highly metastatic potential to liver showed fairly low adhesion activity to the peritoneum compared with KMI2C cells. The mouse peritoneum was found to contain a1-2, a1-3, and a1-4 fucosyltransferases, and adhesion of cancer cells was observed to the cellulose ester membrane, on which partially purified a-fucosyltransferases from mouse peritoneum were immobilized. The adhesion of cancer cells to fucosyltransferase-immobilized membrane was specifically inhibited by the addition of oligosaccharides and glycoproteins, which could serve as substrates for a-fucosyltransferases.
These results indicate the contribution of a-fucosyltransferases to the adhesion of disseminated cancer cells to the peritoneum and support the possibility of antiadhesion therapy of peritoneal dissemination by treatment with substrates for fucosyltransferases.
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