1995 Fiscal Year Final Research Report Summary

STUDY FOR LOCALIZATION OF ONCOGENEIN COLORECTAL CANCER.

Research Project

Project/Area Number	06671262
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Digestive surgery
Research Institution	HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE
Principal Investigator	KAWAKAMI Kazuhiko HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE,SECOND DEPARTMENT OF SURGERY,ASSISTANT, 医学部, 助手 (10252181)
Co-Investigator(Kenkyū-buntansha)	MIYAGAWA Atsuo HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE,PHOTON MEDICAL RESEARCH CENTER,VISITING, 客員教授 BABA Shozo HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE,SECOND DEPARTMENT OF SURGERY,PROFESSOR, 医学部, 教授 (40107818) TERAKAWA Susumu HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE,PHOTON MEDICAL RESEARCH CENTER,PROFESSOR, 光量子医学研究センター, 教授 (50014246)
Project Period (FY)	1994 – 1995
Keywords	ONCOGENE / COLORECTAL CANCER / IN-SITU HIBRIDIZATION / BCL-2
Research Abstract	Bcl is a gene which was supposed to have a relation to apoptosis. In this study, we planned to analyze the localization of bcl-2 mRNA in human colon cancer by in situ RT-PCR method. Lymphnodes contracted with B-cell lymphoma cell were used as a positive control. Bcl-2 positive cells were detected by immuno histochemical staining, but the mRNA of bcl-2 were not detected by in situ RT-PCR method. It was not improved by charging the duration time of proteinase K treatment. The occurance of some degeneration of mRNA was suspected.