1995 Fiscal Year Final Research Report Summary
Experimental study on aggravation in acute pancreatitis and development of new therapy
| Project/Area Number |
06671316
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | St.Marianna University, School of Medicine |
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Principal Investigator |
KOMORIYAMA Hiroyuki St.Marianna University, School of Medicin, Department of Surgery, Lecturer, 医学部, 講師 (70178383)
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| Co-Investigator(Kenkyū-buntansha) |
KUBOTA Sunao St.Marianna University, School of Medicine, Department of Surgery, Assistant Pro, 医学部, 助教授 (20075500)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Acute pancreatitis / Free radicals / Arterial infusion |
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| Research Abstract |
Acute pancreatitis was induced dogs by the retrograde injection of bile into the pancreatic duct. Dogs were divided into two groups, i.e.non-treated control group and treated one in which dogs were administered with a selective thromboxane A2 (TXA2) synthetase inhibitor CV-4151. Concentrations of malondialdehyde (MDA), TXA2 and phospholipase A2 (PLA2) in the treated group were significantly lower than those in the control group, while concentrations of superoxide dismutase (SOD) and 6-keto-prostaglandin F1 did not differ between the two groups. The results demonstrate that oxygen-derived free radicals were generated by the arachidonic acid cascade in the experimental acute pancreatitis model, suggesting that free radicals and several metabolic products would be responsible for disorder of microcirculation and ischemia in the pancreatic tissue. These results also suggested that CV-4151 inhibited the increase in the TXA2 concentration and prevented disorder of microcirculation and ischemia in the pancreatic tissue. Roles of free radicals in the development of acute pancreatitis and effects of the administration of a SOD preparation were studied. Immediately after the induction of acute pancreatitis, a dose of 10,000 u/kg of SOD was administered via the ceriac artery into the animals. Activity levels of MDA,xanthie dase and PL were found to increase in the control group, while suppressed in the experimental group. The survival rate was significantly higher in treated group than control group. These results indicate that SOD could effectively be used for the purpose of treating acute pancreatitis. And treatment of severe acute pancreatitis by arterial infusion of drugs, is much more effective than that by venous injection.
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