1995 Fiscal Year Final Research Report Summary
ESTABLISHMENT AND CHARACTERIZATION OF CULTURED VASCULAR ENDOTHELIAL CELLS DERIVED FROM PATIENTS WITH MOYAMOYA DISEASE
| Project/Area Number |
06671378
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | TOKYO MEDICAL & DENTAL UNIVERSITY,SCHOOL OF MEDICINE |
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Principal Investigator |
AOYAGI Masaru TOKYO MEDICAL & DENTAL UNIVERSITY,SCHOOL OF MEDICINE,ASSOCIATE FROFESSOR, 医学部, 講師 (40134704)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUSHIMA Yoshiharu TOKYO MEDICAL & DENTAL UNIVERSITY,SCHOOL OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 助教授 (20134679)
YAMAMOTO Kiyotaka TOKYO METROPOLITAN INSTITUTE OF GERONTOLOGY,DEPARTMENT OF CELL BIOLOGY,CHIEF RES, 細胞生物部門, 主任研究員 (90073022)
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| Project Period (FY) |
1994 – 1995
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| Keywords | moyamoya disease / vascular endothelial cells / cell culture / muscle, smooth / arterial wall |
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| Research Abstract |
(1) We cultured and established three human vascular endothelial cells derived from patients with moyamoya disease. The endothelial cells were cultured from the capillary of the omentum obtained during bypass surgeries. General biological characteristics of the cells, including proliferative potentials, were investigated and compared with cells from control patients. We have found no difference in the biological charcteristics between cells of moyamoya and control patients presently. We are now investigating other charcteristics, including the permeability and the expression of adhesion molecules of the endothelial cells. The results will be potentially useful in elucidating the mechanism of early development of intimal thickening in moyamoya disease. (2) We histologically examined superficial temporal arteries of moyamoya patients with electron microscopies and immunohistochemistries. Intimal thickenings appeared significantly earlier of age in moyamoya patients than in controls. Migration and proliferation of smooth muscle cells (SMCs) from the media to the intima was often seen in moyamoya patients. Synthetic phenotype of SMCs was obesrved in the thickened intima of moyamoya disease. The results indicate systemic factors that promote migration and proliferation of SMCs in moyamoya disease. (3) We examined the development of intimal thickening after balloon endothelial denudation in rabbit carotid arteries. We found that elastin formation and the expression of tropoelastin gene increased in SMCs entering quiescence after the end of the proliferative phase in the intima. The results will be of help in understanding the mechanisms of formation of multilayred elastic lamina in the thickend intima of moyamoya patients.
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