1995 Fiscal Year Final Research Report Summary
Experimental study of gene therapy for malignant brain tumor
| Project/Area Number |
06671392
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
KURISU Kaoru Hiroshima University School of Medicine Professor, 医学部, 教授 (70201473)
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| Co-Investigator(Kenkyū-buntansha) |
UOZUMI Tooru Hiroshima University School of Medicine Professor, 医学部, 教授 (20028426)
SUGIYAMA Kazuhiko Hiroshima University Medical Hospital Assistant Professor, 医学部・附属病院, 講師 (30243554)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Malignant brain tumor / Gene therapy / Cytokine |
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| Research Abstract |
Fundamental experiments of the gene therapy for malignant brain tumor were performed by transfection of cytokine genes, cell regulation genes and cell structure genes. As for cytokine genes we confirmed antitumor effect to the malignant glioma cells both in vitro and in vivo by transfer of TNF-alpha gene or INF-beta gene so far. In addition of these results, We transferd of G-CSF gene into human glioma cells and then transplanted them into subcutaneous space of nude mouse. As a result of this experiment, nude mice that were transplanted G-SCF gene transfected glioma cells did not from solid tumor and histological evaluation of the G-CSF gene transfected tumor revealed intense invasion of granulocytes into the tumor. We revealed delation or mutation of cell regulation genes such as p21 and p16 in human glioma cells. So transfection these cell regulation genes into human glioma cells was performed. As aresult of transfection, growth suppression of human glioma cell was revealed. This result leads to the possibility that transfer of cell regulation genes may be the candidate of the gene therapy for the malignant glioma. GFAP is one of the cell structure component and the expression of GFAP seems to relate to cell differentiation. In this study, we transfected GFAP gene into medulloblastoma cells, resulting in suppressed cell growth and increased sensitivity to anticancer drugs. These results indicate that chemotherapy to medulloblastoma may be much more effective by transferring GFAP gene into tumor cells.
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