1995 Fiscal Year Final Research Report Summary

Human osteoprogenitor cells in ilium : Isolation and its application for femoral neck fracture

Research Project

Project/Area Number	06671443
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Orthopaedic surgery
Research Institution	NIIGATA UNIVERSITY SCHOOL OF MEDICINE
Principal Investigator	DOHMAE Youichiro NIIGATA UNIVERSITY SCHOOL OF MEDICINE Associate Prof, 医学部, 助教授 (80227701)
Co-Investigator(Kenkyū-buntansha)	TANIZAWA Tatuhiko Niigata University SCHOOL OF MEDICINE Associate Prof, 医学部・附属病院, 講師 (90227231) ENDO Naoto Niigata University SCHOOL OF MEDICINE, 医学部・附属病院, 助手 (10251810) TAKAHASHI Hideaki Niigata University SCHOOL OF MEDICINE Professor, 医学部, 教授 (50018397)
Project Period (FY)	1994 – 1995
Keywords	human bone marrow cell / CFU-F / osteoprogenitor cell / Histomorphometry
Research Abstract	Undifferntiated mesenchymal cells in bone marrow differentiate to form CFU-Fs in vitro, and some of CFU-Fs show osteoblastic features. The effect of growing and aging on the number of CFU-F harvested from ilium in human was examined. The number of ALP positive CFU-Fs was high undr 18 years of age, and gradual decline in the number was observed with aging. Secondly, the effect of BMP-2 on the CFU-F was examined. BMP-2 affected the differentiation of osteoprogenitor cells (ALP positive CFU-F), suggesting that BMP-2 could be one of potent stimulators to differentiate osteoprogenitor cells into osteoblasts.