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1995 Fiscal Year Final Research Report Summary

MECHANISMS AND INHITION OF THE INVASION OF MALIGNANT BONE AND SOFT TISSUE TUMORS THROUGH BASEMENT MEMBRANES

Research Project

Project/Area Number 06671462
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

IWAMOTO Yukihide  KYUSHU UNIVERSITY,MEDICAL DEPT., ASSOCIATE PROFESSOR, 医学部, 助教授 (00213322)

Project Period (FY) 1994 – 1995
KeywordsMALIGNANT TUMOR / BONE AND SOFT TISSUE / METASTASIS / BASEMENT MEMBRANES / INVASION
Research Abstract

Tumor invasion of the basement membranes is a crucial step in the complex multistage process which leads to the formation of metastasis. Tumor cells cross the basement membranes as they initially invade vascular beds during dissemination, and then they penetrate their target tissue. The treatment of human fibrosarcoma cells HT1080 with cAMP analogues and chorela toxin that increase the intra-cellular cAMP concentrations induces the expression of Timp-1 and Timp-2 both at the mRNA and the protein levels. This induction of Timps correlated well with type IV collagenolytic activity, invasiveness and metastatic potential of HT1080 cells.
We developed a quantitative assay for assessing in vivo tumor angiogenesis using basement membrane extracts (Matrigel). Nude mice are injected s.c. with liquid Matrigel mixed with HT1080 cells. Since Matrigel forms a solid gel rapidly at body temperature, the gel containing tumor cells can be removed immediately, and the processed for histological studies. Tumor angiogenesis was monitored quantitatively by measuring both the number and total area of neovessels present in the gels using an image analyzer.

  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Y. Iwamoto, et al.: "In vitro assay of the invasive potential of malignant bone and sofy tissue tumors through basement membranes." International Orthop.18. 240-247 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Okuyama, Y. Iwamoto, et al.: "The inhibition of the invasion of human fibrosarcoma cells by cyclic AMP analogues and cholera toxin." The Cancer J.7:. 150-157 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Miyashi, Y. Iwamoto, et al.: "The effects of cisplatin on the epiphyseal growth plate." International J.Oncology. 4:. 1105-1109 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Fujita, Y. Iwamoto, et al.: "Drug-induced endothelial iniury potentiates lung colonization in a murine fibrosarcoma model." International J. Oncology. 4:. 1305-1310 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Iwamoto, et al.: "Role laminin on the invasion of HT1080 human fibrosarcoma cells through basement membranes." Transactions of the 40th Annual Meeting of Orthopaedic Research Society.758 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Okuyama, Y. Iwamoto, et al.: "Cyclic AMP analogues and cholera toxin inhibit the invasion of human fibrosarcoma cells." Transactions of the 40th Annual Meeting of Orthopaedic Research Society.759 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Tanaka, Y. Iwamoto, et al.: "Cyclic AMP-induced synthesis of tissue inhibitor of metalloproteinases suppress the in vasive potential of the human fibrosarcoma cell line HT1080." Cancer Res.55:. 2927-2935 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Tanaka, Y. Iwamoto, et al.: "The establishment and characterization of a peripheralneuroepithelioma cell line in soft tissue of extremity." Laboratory Investigation. 72:. 237-248 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Ito, Y. Iwamoto, et al.: "A quantitative assay using basement membraneextracts to study tumor angiogenesis in vivo." International J. Cancer.(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Naka, Y. Iwamoto, et al.: "Osteosarcoma versusmalignant fibrous histiocytoma of bone in patients older than 40 years." Cancer. 76:. 972-984 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Iwamoto, et al.: "Three-dimentional MRI reconstruction of musculoskeletal tumors. A preliminary evaluation of 2 cases." Acta Orthop. Scand.66:. 80-83 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Iwamoto, et al.: "Role of MR imaging in the diagnosis of alveolar soft part sarcoma:A report of 10 cases." Skeletal Radiology. 24:. 267-270 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本幸英、伊藤康正。: "腫瘍血管新生の分子機構とその制御" BIO clinica. 11巻. 60-62 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y. Ito, Y. Iwamoto, et al.: "A new method using basement membraneextracts to stuidy tumor angiogenesis in vivo." Transactions of the 41st Annual Meeting of Orthopaedic Research Society.208 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Tanaka, Y. Iwamoto, et al.: "Induction of TIMPs by elevation of intracellular cyclic AMP concentration in hibits the invasiveness of human fibrosarcoma cells." Transactions of the 41st Annual Meeting of Orthopaedic Research Society.278 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Tanaka, Y. Iwamoto, et al.: "Comparison of the effects of intra-arterial and intravenous cisplatin on foot tumors and survival of rats bearing lung metastases." Transactions of the 41st Annual Meeting of Orthopaedic Research Society.437 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Naka, Yukihide Iwamoto, et al.: Human Pathology. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本幸英、小田義直。: "悪性線維性組織球腫(MFH)" Orthopaedics Today. (印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本幸英。: "悪性形質" 成形外科:. 63巻. 1786 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中馬広一、岩本幸英、ほか。: "再発、再燃した悪性骨軟部腫瘍に対する化学療法の検討" 整形外科と災害外科:. 43巻. 765-767 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本幸英: "原発性骨悪性腫瘍切除後の再建に対する同種骨の応用." 骨関接靱帯:. 7巻. 343-348 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本幸英。: "四肢の転移性骨腫瘍に対する手術療法:集学的治療の中での位置付けについて." Therapeutic Research:. 15巻. 419-423 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本幸英。: "同種骨移植による再建: Massive segmental allograft" 整形・災害外科. 38巻. 825-831

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本幸英。: "日本における同種骨移植の現状" 整形外科. 63巻. p1786 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本幸英、小田義直。: "新版図説臨床整形外科講座13巻「骨軟部腫瘍および類似疾患」、(腫瘍と間違えやすい軟部病変)" メディカルレビュー社、東京, 350(319-326) (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩本幸英。: "OS NOW 18 :骨腫瘍の診断と治療(肺転移に対する治療-肺転移の抑制、治療)" メディカルレビュー社、東京, 241(236-241) (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Okuyama, Y.Iwamoto, et al.: "The inhibition of the invasion of human fibrosarcoma cells by cyclic AMP analogues and choleratoxin." The Cancer J.7. 150-157 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Miyoshi, Y.Iwamoto, et al.: "The effects of cisplatin on the epiphyseal growth plate." International J.Oncology. 4. 1105-1109 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Fujita, Y.Iwamoto, et al.: "Drug-induced endothelial injury potentiates lung colonization in a murine fibrosarcoma model." International J.Oncology. 4. 1305-1310 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Iwamoto, et al.: "In vitro assay of the invasive potential of malignant bone and soft tissue tumors through basement membranes." Inernational Orthop.18. 240-247 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Iwamoto, et al.: "Role of laminin on the invasion of HT1080 human fibrosarcoma cells through basement membranes." Transactions of the 40th Annual Meeting of Ortopaedic Research Society. 758 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Okuyama, Y.Iwamoto, et al.: "Cyclic AMP analogues and cholera toxin inhibit the invasion of human fibrosarcoma cells." Transactions of the 40th Annual Meeting of Orthopaedic Research Society. 759 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Tanaka, Y.Iwamoto, et al.: "The establishment and characterization of a peripheral neuroepithelioma cell line in soft tissue of extremity." Laboratory Investigation. 72. 237-248 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Tanaka, Y.Iwamoto, et al.: "Cyclic AMP-induced synthesis of tissue inhibitor of metalloproteinases suppress the in vasive potential of the human fibrosarcoma cell line HT1080." Cancer Res.55. 2927-2935 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Naka, Y,Iwamoto, et al.: "Osteosarcoma versus malignant fibrous histiocytoma of bone in patients older than 40 years." Cancer. 76. 972-984 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Iwamoto, et al.: "Three-dimentional MRI reconstruction of musculoskeletal tumors. A preliminary evaluation of 2 cases." Acta Orthop.Scand.66. 80-83 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Iwamoto, et al.: "Role of MR imaging in the diagnosis of alveolar soft part sarcoma : A report of 10 cases." Skeletal Radiology. 24. 267-270 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Ito, Y.Iwamoto, et al.: "A new method using basement membrane extracts to study tumor angiogenesis in vivo." Transactions of the 41st Annual Meeting of Orthopaedic Research Sjociety. 208 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Tanaka, Y.Iwamoto, et al.: "Induction of TIMPs by elevation of intracellular cyclic AMP concentration inhibits the invasiveness of human fibrosarcoma cells." Transactions of the 41st Annual Meeting of Orthopaedic Research Society. 278 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Tanaka, Y.Iwamoto, et al.: "Comparison of the effects of intra-arterial and intravenous cisplatin on foot tumors and survival of rats bearing lung metastases." Transactions of the 41st Annual Meeting of Orthopaedic Research Society. 437 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Ito, Y.Iwamoto, et al.: "A quantitative assay using basement membrane extracts to study tumor angiogenesis in vivo." International J.Cancer. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Naka, Yukihide Iwamoto, et al.: "Proliferative activities in conventional chordoma. A clinicopathologic, DNA flow cytometric and immunohistochemical analysis of 17 cases with special reference to anaplastic chordoma showing a diffuse proliferation and nuclear atypia." Human Pathology. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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