Etiology of Cleft Palate and its gene therapy

1995 Fiscal Year Final Research Report Summary

• Project/Area Number: 06671463
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: NAGASAKI UNIVERSITY
• Principal Investigator: FUJII Tohru Nagasaki Univ.Sch.of Med.Dept.of Plastic & Reconstructive Surgery Professor, 医学部, 教授 (60136661)
• Co-Investigator(Kenkyū-buntansha): NAMBA Hiroyuki Nagasaki Univ.Sch.of Med.Dept.of Cell Physiology Associate Professor, 医学部・原研発症予防部門, 助教授 (80237635) ; YAMASHITA Shunichi Nagasaki Univ.Sch.of Med.Dept.of Cell Physiology Professor, 医学部・原研発症予防部門, 教授 (30200679)
• Project Period (FY): 1994 – 1995
• Keywords: Parathyroid hormone related protein / Transforming growth factor beta / Anti-sense / S-oligo-DNA / Gene therapy / Cleft palate

Research Abstract

Normal palate develops through the vertical growth, the bilateral elevation and fusion. We investigated expression of TGT beta and PTHrP in this fine process of the palate development using immunohistochemistry and in situ hybridization. Expression of both factors were recognized in the epithelium of palatal shelves and the mesenchymal condensation for bone. Concerning administration of Vitamine A induce cleft palate in pregnant rodents, reduction of PTHrP expression in embryonic facial tissue and decrease of DNA synthesis were also recognized from Vitamine A administration. However we could not show a good result of intra-amniotic administration of anti-sense S-oligo DNA for PTHrP.Although FITC-oligo was confirmed to be taken to the embryo, no remarkable change was observed. From now on, we would use organ culture, and attempt to elucidate the cleft-palate etiology.

Research Products

• [Publications] Nakashima M et al: "Expression of parathyroid hormone-related peptide in human thyroid tumors." J Pathol. 175. 227-236 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Namba H et al: "Radiation-induced G1 arrest is selectively mediated by the p53-WAF1/Cip1 pathway in human thyroid cells." Cancer Res. 55. 2075-2080 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tsukazaki T et al: "Expression of parathyroid hormone-related protein in rat articular cartilage" Cal Tissue Int. 57. 196-200 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Akino K et al: "Antisense inhibition of parathyroid hormone-related peptide gene expression reducesmalignant pituitary tumor progression and metastasis in the rat" Cancer Res. 56. 77-86 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ozeki S et al: "Evidence implicating parathyroid hormone-related peptide in vascular stenosis: increasedgene expression observed in the intima of injured rat carotid arteries and human restenotic coronary lesions" Arterioscler Thromb. (in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yano H et al: "Involvement of c-Fos proto-oncogine during palatal fusion and interdigital space formation in the rat" Dev Growth Differ. (in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yamashita S et al: "role of parathyroid hormone-related peptide in the pathogenesis on pituitary adenomas." Oncogenesis and Molecular Biology on Pituitary tumors, 10 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nakashima M,Ohtsuru A,Luo W T,Nakayama T,Enomoto H,Usa T,Kiriyama T,Ito M,Nagataki S,Yanashita S,Sekine I: "Expression of parathyroid hormone-related peptide in human thyroid tumors." J Pathol. 175. 227-236 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Namba H,Hara T,Tukazaki T,Migita K,Ishikawa N,Ito K,Nagataki S,Yamashita S: "Radiation-induced G1 arrest is selectively mediated by the p53-WAF1/Cip1 pathway in human thyroid cells." Cancer Res. 55. 2075-2080 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tsukazaki T,Ohtsuru A,Enomoto H,Yano H,Motomura K,Ito M,Namba H,Iwasaki K,Yamashita S: "Expression of parathyroid hormone-related protein in rat articular cartilage" Cal Tissue Int. 57. 196-200 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hirano A,Akita S,Fujii T: "Craniofacial deformities associated with juvenile hyperthyroidism." Cleft Palate Craniofac J. 32. 328-333 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Arakaki M,Yamashita S,Mutaf M,Naito S,Fujii T: "Onlay silicon and hydroxyapatite-tricalciumphosphate composite (HAP-TCP) blocks interfere with nasal bone growth in rabbits." Cleft Palate Craniofac J. 32. 282-289 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Akino K,Ohtsuru A,Yano H,Ozeki S,Namba H,Nakashima M,Ito M,Matsumoto T,Yamashita S: "Antisense inhibition of parathyroid hormone-related peptide gene expression reduces malignant pituitary tumor progression and metastasis in the rat." Cancer Res. 56. 77-86 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ozeki S et al: "Evidence implicating parathyroid hormone-related peptide in vascular stenosis : increased gene expression observed in the intima of injured rat carotid arteries and human restenotic coronary lesions." Arterioscler Thromb. (in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yano H,Ohtsuru A,Ito M,Fujii T,Yamashita S: "Involvement of c-Fos protooncogine during palatal fusion and interdigital space formation in the rat." Dev Growth Differ. (in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yano H,Yoshimoto H,Ohtsuru A,Ito M,Yamashita S,Fujii T: "Characterization of rat embryonic palatal mesenchymal cells." Cleft Palate Craniofac J. (in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamashita S,Akino K,Ohtsuru A: "Role of parathyroid hormone-related peptide in the pathogenesis on pituitary adenomas." Oncogenesis and Molecular Biology on Pituitary tumors. 1996.
  • Description: 「研究成果報告書概要(欧文)」より