1995 Fiscal Year Final Research Report Summary
Investigation of nitric oxide for pain transmission mechanism at spinal cord
| Project/Area Number |
06671505
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | University of Tsukuba |
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Principal Investigator |
SAITO Shigeyuki Univ.Tsukuba., Inst.Clin.Med., Assist P., 臨床医学系, 講師 (20186935)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIKAWA Toshiaki Univ.Tsukuba., Inst.Clin.Med., Assist P., 臨床医学系, 講師 (50156048)
YAMAGUCHI Hiroshi Univ.Tsukuba., Inst.Clin.Med., Assist P., 臨床医学系, 講師 (60220274)
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| Project Period (FY) |
1994 – 1995
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| Keywords | nitric oxide / nitric-oxide synthase / spinal cord / Rat / pain / L-NAME |
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| Research Abstract |
We investigated the formalin pain induced development of nitric oxide synthase containing neuron at same side spinal cord. It is not clear whether does pain induce nitric oxide at the spinal cord by lower leg pain.
Twenty four rats were used for this study. The rat was anesthetized by pentobarbitol intraperitonealy. Then ventilated artificially through the theoacheostomy using muscle relaxant. Femoral vein and artery was cannulated by P-10 catheter for blood pressure monitoring, blood gas analysis and drug administration.
Thoracic spin (Th 10-12) was laminectomized and nitric oxide electrode was inserted to spinal canal for nitric oxide measurement. SNP was administered intravenously for confirm electrode function. Lower leg was pinched by pean for 5 seconds. The response of nitric oxide production was recorded by pen recorder with arterial pressure. Three times trial was performed for measurement. The nitric oxide response was slightly decreased then recovered to baseline.
Then nitric oxide synthase blocker L-NAME (0.3,3,30mg/kg) was infused. The same procedure was performed. L-NAME depressed nitric oxide production by electrode. The pain induced blood pressure response was maintained with the same response of nitric oxide production by electrode.
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