1995 Fiscal Year Final Research Report Summary
THE ISCHEMIC CHANGES IN THE MITOCHONDRIA CALCIUM CONCENTRATION IN THE RAT BRAIN, HEART, AND LIVER
| Project/Area Number |
06671547
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | KYOTO PREFECTURAL UNIVERSITY OF MEDICINE |
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Principal Investigator |
SEKIMOTO Miho KYOTO PREFECTURAL UNIVERSITY OF MEDICINE, DEPARTMENT OF ANESTHESIOLOGY, ASSISTANT PROFESSOR, 医学部, 助手 (00244583)
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| Co-Investigator(Kenkyū-buntansha) |
OHMORI Misako KYOTO PREFECTURAL UNIVERSITY OF MEDICINE, DEPARTMENT OF ANESTHESIOLOGY, ASSISTANT PROFESSOR, 医学部, 助手 (60264777)
KOBAYASHI Atsuko KYOTO PREFECTURAL UNIVERSITY OF MEDICINE, DEPARTMENT OF ANESTHESIOLOGY, ASSISTANT PROFESSOR, 医学部, 助手 (70264778)
KINOSHITA Takashi KYOTO PREFECTURAL UNIVERSITY OF MEDICINE, DEPARTMENT OF ANESTHESIOLOGY, ASSISTANT PROFESSOR, 医学部, 助手 (80264779)
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| Project Period (FY) |
1994 – 1995
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| Keywords | CALCIUM / BRAIN / HEART / ISCHEMIA / LIVER / MITOCHONDRIA |
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| Research Abstract |
The intracellular calcium is stored mostly in the mitochondria and SR. The calcium in SR is utilized for the control of the intracellular concentration of calcium in the steady-state. The critical increase in calcium concentration in the ischemic state is thought to be induced by the calcium release from mitochondria. Therefore, we investigate the change in calcium concentration in mitochondria in the schemic state in order to observe the initial change in the ischemic cellular damage.
We tried to separate mitochondria from rat brain, heart, and liver, according to Hogeboom's method, Palmer's method, and Inoue's method, respectively, and to measure the intracellular calcium concentration in the ischemic condition by using calcium fluorescent agents.
However, we were not able to separate pure mitochondrial samples enough to have high respiratory activity and to be stained by these fluorescent agents unfortunately. This might be due to the mitochondria separation methods we had employed, and due to our unskillful treatments. Further investigations have to be performed to elucidate the initial ischemic change of calcium in mitochondria.
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