1996 Fiscal Year Final Research Report Summary
Protection of Spermatogenetic damage induced by anti-cancer drug using LH-RH agonist.
| Project/Area Number |
06671568
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Urology
|
|---|
| Research Institution | University of Tsukuba |
|---|
Principal Investigator |
TAKESHIMA Hitoshi Inst.of Clinic.Med., University of Tsukuba Assistant prof., 臨床医学系, 講師 (00197287)
|
|---|
| Project Period (FY) |
1994 – 1996
|
| Keywords | Spermatogenesis / Spermatogenetic impairment / Anti-cancer drug / Doxorubicin / LH-RH agonist / Leuprorelin / Johnsen's score / Image cytometry |
|---|
| Research Abstract |
INTRODUCTION AND OBJECTIVES : Spermatogenetic disorder in male patients after successful anticancer chemotherapy is one of important issues. Endocrinological suppression of germ cell division could protect spermatogenesis from anticancer drugs. In this study, LH-RH agonist (Leuprorelin) were used and evaluated its protective effect on spermatogenetic damage induced doxorubicin.
METHODS : SD strain malerats were divided into four groups. 1) control group, 2) LH-RH agonist (subcutaneous injections, total dose 9mg/kg) administered group, 3) doxorubicin (intraperitoneal injections, total dose 7.5mg/kg) administered group and 4) LH-RH agonist (subcutaneous injections, total dose 9mg/kg) and doxorubicin (intraperitoneal injections, total dose 7.5mg/kg)administered group. Evaluation was made by measuring body and testicular weights, counting the Johnsen's score, and DNA image analysis : DNA content measurement (%1C,%2C,%4C) by image cytometry.
RESULTS : In the group administtered adriamycin, the testicular weigth was 1.5<plus-minus>0.2mg, the Johnsen's score was 4.4<plus-minus>1.2, and image analysis revealed %1C : 33.8<plus-minus>9.2, %2C : 43.9<plus-minus>16.3 and %4C : 5.0<plus-minus>4.4 respectively. In both LH-RH agonist and adriamycin administered group, the testicular weight was 1.3<plus-minus>0.2mg, the Johnsen's score was 5.9<plus-minus>1.6, and image analysis disclosed %1C : 46.9<plus-minus>15.0, %2C : 28.4<plus-minus>13.3 and %4C : 8.8<plus-min
CONCLUSIONS : Significant prophylactic effect was demonstrated on Johnson's score (p<0.05) and by image analysis ; %1C (p<0.005), %2C (p<0.005) and %4C (p<0.05). Based on these results, it is concluded that LH-RH agonist could protect spermatogenesis from the damage induced by doxorubicin.
|
