1996 Fiscal Year Final Research Report Summary
Alteration of rpidermal growth factor gene and p53 gene in the oncogenesis of urothelial tumors.
| Project/Area Number |
06671581
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kanazawa University |
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Principal Investigator |
KUNIMI Kazuto University Hospital Kanazawa University Assistants, 医学部・付属病院, 助手 (40251954)
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| Project Period (FY) |
1994 – 1996
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| Keywords | bladder cancer / ureteral cancer / clomerase / MT-MMP / mitotic ability / invasiveness |
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| Research Abstract |
1. Telomerase activity
Telomerase activity is highly elevated in human cancer cells which possess unlimited life span, in spite that somatic cells have little or no activity. Telomerase activity was investigated using PCR-based telomeric repaet amplification assay in 22 cases of urothelial (baldder and ureter) tumors. No activity was detected in normal urothelial tissues. In contrast, all of the tumor tissue showed telomerase activity with different levels among the tumor samples. No correlation was found between the activity level and pathological grading/clinical staging.
2. Membrane-type matrix metalloproteinase (MT-MMP,type1,2,3)
MT-MMPs (type1,2,3) are responsible for local invasion of cancer cells into the stromal space. Expression levels of MT-MMPs wer studied using RT-PCR and immunostaining in urothelial tumoes and the normal counterpart. Type1 MT-MMP expressions were weak in both normal and tumor tissues with no significance. Type2 and 3 MT-MMP showed significantly high expression in tumor tissue compared with the normal tissues, and multiple occurring tumors had higher expression level than single occurring tumors. Type 1 MT-MMP expressions were higher in invasive type tumors that in superficial type.
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