1995 Fiscal Year Final Research Report Summary
MECHANISM OF ANDROGEN DEPENDENT MORPHOGENESIS OF PROSTATE GLAND : ROLE OF GROWTH FACTORS IN ORGAN CULTURE.
| Project/Area Number |
06671583
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | AICHI CANCER CENTER |
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Principal Investigator |
SUGIMURA Yoshiki AICHI CANCER CENTER,RESEARCH FELLOW, 研究所, 研究員 (90179151)
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| Co-Investigator(Kenkyū-buntansha) |
HIOKI Takuichi DEPARTMENT OF UROLOGY,AICHI CANCER CENTER,CLINICAL STAFF, 泌尿器科部, 医長
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| Project Period (FY) |
1994 – 1995
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| Keywords | PROSTATE / ORGAN CULTURE / ANDROGEN / GROWTH FACTOR / DUCTAL MORHOGENESIS / KGF / EGF |
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| Research Abstract |
To investigate the androgenic signal communicating from androgenreceptor-positive mesenchyme to the epithelium, the functions of keratinocyte growth factor (KGF) on the prostate branching morphogenesis was examined with serum-free prostate organ culture system in the rat ventral prostate (VP) gland. Organ culture of rat neonatal VP's were incubated on the Millipore filters under serum and growth factor-free conditions. When testosterone (T,10^<-8>M) was added to the basal media, VP's grew and underwent branching morphogenesis similar to that in situ. Neutralizing antibody to KGF,inhibited androgen-stimulated VP growth after 6 days in culture. When KGF was added to the media instead of T,it stimulated VP growth to a levels of 50% in branching ends of that which is possible with an optimal dose of Testosterone. KGF is a member of the fibroblast growth factor family and is suggested to be produced by mesemchymal/fibroblast cells and is able to act sole on epithelial cells. In this study.we demonstrated that KGF play an important role as a paracrine mediator on the mesenchymal-epithelial interaction in the branching morphogenesis required for prostate development.
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[Publications] Sugimura Y,Hioki T,Hayashi N,Kawamura J,Rubin JS,Aaronson SA,Cunha GR: Mesenchymal-epithelial interactions in prostate growth : Role of growth factors as androgenic signals. in Imai, K., Shimazaki, J., Karr, J.P.(eds) : Fundametal approaches to the Diagnosis and Treatment of Prostate Cancer and BPH. New York, Adenine Press, 97-102 (1994)
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