1995 Fiscal Year Final Research Report Summary
BASIC AND CLINICAL INVESTIGATION ON FIBROBLAST GROWTH FACTORS AND FIBROBLAST GROWTH FACTOR RECEPTORS IN HUMAN PROSTATE
| Project/Area Number |
06671590
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
NAKAHARA Mitsuru HIROSHIMA UNIVERSITY SCHOOL OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (70155802)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Human Prostate / Fibroblast Growth Factors / Fibroblast Growth Factor Receptors |
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| Research Abstract |
Expression of fibroblast growth factor family (FGFs) and FGF receptors (FGFRs) were investigated in human benign prostatic hypertrophy (BPH) and prostate cancer using reverse transcription polymerase chain reaction (RT-PCR) method and immunohistochemical method.
Regarding the results, primary cultured prostatic epithelial cells which contained more than 90% of epithelial cells expressed the mRNA for FGF1, FGF2, FGFR1 and FGFR2 (IIIb) and primary cultured prostatic stromal cells which contained 100% of stromal cells expressed the mRNA for FGF1, FGF2, FGF7 and FGFR1 in BPH.LNCaP cells which are androgen responsive and non-metastatic expressed the mRNA for FGF1, FGFR1 and FGFR2 (IIIb). PC3 cells which are androgen independent and highly metastatic expressed the mRNA for FGF1, FGF2, FGFR1 and FGFR2 (IIIc). Immunoreactive FGF1 was detected in epithelial cells, whereas FGF2 was detected in stromal cells and FGFR1 was not identical in BPH tissues. However, immunoreactive FGF1, FGF2 and FGFR1 were detected in moderately differentiated adenocarcinoma of the prostate.
These results suggested that FGF1, FGF2 and FGF7 play some role on epithelial-stromal interaction through FGFR1 and FGFR2 in human BPH.In prostate cencer, expression of FGF2, FGFR1 and FGFR2 (IIIc) may be involved in malignant progression.
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