1995 Fiscal Year Final Research Report Summary
Flow cytometric DNA analysis of contralateral testicular spermatogenesis in experimental unilateral testicular torsion
| Project/Area Number |
06671591
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
TAKIHARA Hiroshi Yamaguchi Univ Sch Med, Associate Professor, 医学部, 助教授 (50144936)
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| Co-Investigator(Kenkyū-buntansha) |
BABA Yoshikazu Shuto General Hospital, Chief Division of Urol, 部長 (60208717)
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| Project Period (FY) |
1994 – 1995
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| Keywords | torsion / germ cell DNA / fertility rate / testicular ischemia |
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| Research Abstract |
It hasbeen posturated that unilateral testicular torsion causes damages to the octralateral testis and concequently reduces fertility, and that detorsion of the twisted testis performed within a critical time might prevent contralateral testicular damages. The purpose of this study is to investigate whether the time from torsion to detorsion affects the spermatogenesis of the contralateral testis or fertility in prepubertal rats using flow cytometry and fertility test. Thirty-five-day-old male SD rats were diveded into 4 groups which underwent sham-operation group (1), 4 hours' duration of unilateral testicular torsion (2), 8 hours' duration of testicular torsion (3), and 12 hours' testicular torsion (4). Five weeks after operation, fertility and contralateral spermatogenesis by flow cytometry weer evaluated.
The results showed that both contralateral testicular spermatogenesis and fertility were not affected by the duration of 4 hours, and that contralateral testicular spermatogenesis was reduced with fertility not being affected by the duration of 8 hours, and that both contralateral testicular spermatogenesis and fertility were reduced by the duration of 12 hours in experimental unilateral testicular torsion of prepubertal rats.
It is concluded that unilateral testicular torsion causes damages to the contralateral testicular function consequantly reducing fertility at puberty, when torsion of the unilateral testis is kept for more than 8 hous.
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