1995 Fiscal Year Final Research Report Summary
Sutudy of The Effect of BCG-Fibronectin-Bladder Tumor Cell Interraction on Antitumor Activity
| Project/Area Number |
06671620
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Toho University |
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Principal Investigator |
KURODA Kanami Toho University, School of Medicine, Assistant, 医学部, 助手 (70192044)
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| Project Period (FY) |
1994 – 1995
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| Keywords | Bladder tumor cell / BCG / Fibronectin / Cytostatic activity / Internalization / Degradation |
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| Research Abstract |
BCG inhibited T-24 cells growth. As attachment and ingestion of BCG by T-24 cells were abrogated by pretreatment of BCG with polyclonal rabbit anti-BCG antibody, the effect of this pretreated BCG on T-24 cells growth was investigated. Pretreatment of BCG with polyclonal anti-BCG antibody abrogated the cytostatic activity of BCG.This results demonstrate that BCG-FN-bradder tumor cell interraction may play an important role in antitumor activity.
Both BCG internalization in vivo and BCG degradation in vitro were not confirmed. As rat was used for the internalization assay, this negative data may be related to no sensitivity of rat to BCG.Furthermore, rat may not be appropriate model. Assy method is required improvement.
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