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1995 Fiscal Year Final Research Report Summary

Coculture of preimplantation embryo with an immortalized human oviductal epithelial cells

Research Project

Project/Area Number 06671654
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionTottori University, Faculty of Medicine

Principal Investigator

HARADA Tasuku  Tottori Univ.・Dept.Obstet.Gynecol., Assistant Professor, 医学部・附属病院, 講師 (40218649)

Co-Investigator(Kenkyū-buntansha) ONOHARA Yoshimasa  Tottori Univ.・Dept.Obstet.Gynecol., Assistant Professor, 医学部, 助手 (00240862)
TANIKAWA Masahiro  Tottori Univ.・Dept.Obstet.Gynecol., Assistant Professor, 医学部・附属病院, 助手 (90236688)
TERAKAWA Naoki  Tottori Univ.・Dept.Obstet.Gynecol., Professor, 医学部, 教授 (90163906)
Project Period (FY) 1994 – 1995
KeywordsOviductal epithelial cells / immortalize / basis fibroblast growth factor / FGF receptor 1
Research Abstract

We have tried establish an immortalized cell line of human tubal epithelium. It is not possible to obtain immortalized cells after repeated experiments. We also have performed experiments using preimplantation embryos. We determined the expression of fibroblast growth factor (FGF) -2 and the FGF receptor 1 (FGFR-1) mRNA in the mouse embryos and in epithelial cells of the uterine endometrium using reverse transcription-polymerase chain reaction (RT-PCR). No FGF-2 mRNA was detected in mouse embryos but FGFR-1 mRNA was expressed at the blastocyst stage. These results suggest importance of FGFR-1 in preimplantation development of embryo. To examine the mechanism of FGFR-1 expression, the arrangement of exons and introns encoding 5'-side of murine FGFR-1 gene was mapped. A large intron with a size of 14kb was identified between exon 1 and 2. All subtypes of FGFR-1 was obserbed to be generated through alternative aplicing. Furthermore, we characterized a transcription element of this gene. The basal promoter element was mapped to the 5'-flanking region from -89 to -43. The DNase I protection assay and gel shift analysis revealed that a nuclear protein could bind to the nucleotide sequence from -62 to -42. In conclusion, FGF-2 produced by the female reproductive tract may exert paracrine effects on preimplantation embryos. FGFR-1 gene has an unique genomic structure. The 5'-flanking region from -62 to -42 plays a pivotal role in FGFR-1 gene expression.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Saito H: "Mapping of a transcviption clement cvitical for expression of the fibroblast growth facfor receptor 1 gene" Biochem Biophys Res Commun. 198. 1020-1026 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Harada T: "Murine fibroblast growth factor receptor 2 genc generates mucltigle messenger RNAs containing two open reading framesvia alternative splicing" Biochem Biophys Res Commun. 205. 1057-1063 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroshi Saito: "Mapping of a transcription element critical for expression of the fibroblast growth factor receptor 1 gene" Biochemical and Biophysical Research Communications. 198(3). 1020-1026 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tasuku Harada: "Murine fibroblast growth factor receptor 1 gene generates multiple messenger RNAs containing two open reading frames via alteernative splicing" Biochemical and Biophysical Research Communications. 205(2). 1057-1063 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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