1995 Fiscal Year Final Research Report Summary
The Factors Involved Invasive Ability of Endometrial Carcinoma Cells (Studies of Concerning Invasion, Metastasis of Endometrial Cancer)
| Project/Area Number |
06671669
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Sapporo Medical University |
|---|
Principal Investigator |
KUDO Ryuichi Medical School, Sapporo Medical University Professor, 医学部, 教授 (70045409)
|
|---|
| Project Period (FY) |
1994 – 1995
|
| Keywords | invasive ability / endometrial cancer / E-cadherin / invasion assay / type IV collagenase / ultrastructure / differentiation |
|---|
| Research Abstract |
A.The in vitro invasive ability, the expression of cell adhesion molecule E-cadherin, activity of matrix metalloproteinase (MMP) and K-ras point mutation were investigated in several human endometrial carcinoma cell lines.
1) In vitro invasive abilities of endometrial carcinoma cell lines depend on the degree of cell differentiation and the origin of cell lines. A poorly-differentiated carcinoma cell line and a cell line derived from metastatic lymph node, or ascitis were more invasive than moderately and well-differentiated cell lines.
2) Immunohistochemically, less invasive cell lines expressed E-cadherin strongly, where as a highly invasive cell line expressed E-cadherin weakly.
3MMP-2 was secreted in serum-free conditioned medium of all cell lines. In an invasive cell line, the activity of MMP-2 was stronger than in other cell lines. And the activity of MMP-9 was detected in most invasive cell line.
4) K-ras point mutation was not correlated with in vitro invasiveness of the tumor cells.
B.The changes of the in vitro invasive ability after administration of anti E-cadherin antibody (HECD-1) were investigated in above cell lines.
1) Immunohistochemically, expression of E-cadherin of less invasive cell lines changed to weak expression after administration of HECD-1.
2) The invasive ability of less invasive cell lines increased after administration of HECD-1. However, that ability of highly invasive cell line was not changed even though these treatment.
3) The ultrastructural features of less invasive cell lines changed to the similar findings of a highly invasive cell line after administration of HECD-1. These findings involved the decreasing cellular connection, pseudopolar cellular projection in invasing through matrigel and numerous microvilli.
|
