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1995 Fiscal Year Final Research Report Summary

The role of EGF and TGFalpha-EFGR in estrogen effect on endometrial cancinogenesis.

Research Project

Project/Area Number 06671673
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionYokohama City University

Principal Investigator

TAKAHASHI Tsuneo  Yokohama City University, School of Medicine, Assistant Professor., 医学部, 講師 (60179497)

Co-Investigator(Kenkyū-buntansha) TAGA Michiyoshi  Yokohama City School of Medicine, Associate Professor., 医学部, 助教授 (00107682)
Project Period (FY) 1994 – 1995
KeywordsESTROGEN / ENDOMETRIUM / EGF / TGF-alpha / EGF RECEPTOR
Research Abstract

The in vivo studies demonstrate the EGF is an important autocrine and/or paracrine mediator of estrogen action on uterus. An antibody specific for EGF significantly inhibited estrogen action on uterus. Estrogen induces the expression of TGFalpha mRNA in the mouse uterus in a dose- and time-dependent manner. The up-regulation of TGFalpha transcripts occurs predomonantly in the uterine epithelial cells. RIA and Western blot analysis demonstrate that immunoreactive TGFalpha protein is secreted at high levels into mouse uterine luminal fluid after estrogen treatment. The induction of uterine TGFalpha mRNA is specific to estrogen ; nonestrogenic sterids did not induce expression. Antibody specific to TGFalpha significantly reduces estrogen-mediated uterine growth, which supports the concept that TGFalpha is a mitogen for the reproductive tract. Analysis of TGFalpha/EGF receptor by binding, affinity labeling, and phosphorylation studies indicates that functional receptors are present in the mouse uterus after estrogen exposure. Thus, our data support a physiological role for EGF/TGFalpha and its receptor pathway in the female mouse reproductive tract.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Takahashi, T: "Transforming growth factor β_1,β_2 and β_3 messenger R&A and protein expression in mouse uterus and vigina during estrogen-induced grouth." Cell. Growth & Differentiation. 5. 919-935 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高橋恒男: "マウス子宮・膣におけるTGFβ_1,_2&3の発現の検討" 日内分泌誌. 70. 714 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高橋恒男: "マウス子宮・腔におえるエストロゲン(E)調節遺伝子の発現検討" 日産婦誌. Suppl.S404 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高橋恒男: "エストロゲンの作用とtransforming growth factor(TGF)" エンドメトリオージス研究会会誌. 16. 8-13 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 杉浦賢: "マウス子宮内膜癌発生過程におけるテネイシンの発現" 日内分泌誌. 71. 813 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahashi, T., Eitzman, B., Bossert, N.L., Walmer, D., Sparrow, K., Flanders, K.C., McLachlan, J., and Nelson, K.G.: "Transforming growth factor beta1, beta2, and beta3 messenger RNA and protein expression in mouse uterus and vaging during estrogen-induced growth : A comparison to other estrogen-regulated genes." Cell Growth & Differentiation. 5. 919-935 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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